Van den Berg F M, Van Amstel P J, Janse C J, Meis J F, Mons B
Department of Pathology, University of Amsterdam, The Netherlands.
Histochem J. 1991 Mar;23(3):109-15. doi: 10.1007/BF01047455.
A highly sensitive non-radioactive DNA in situ hybridization procedure is described that enables detection and unequivocal identification of various developmental stages of human and rodent malaria parasites. Using biotinylated species-specific DNA probes, erythrocytic parasites can be specifically stained in blood smears. Similarly exoerythrocytic stages can be visualized in cell culture and in sections of paraffin-embedded liver. In blood smears, the hybridization procedure provides a rapid detection of (low) parasitemia and species-determination for experienced microscopists at 100 to 400x magnification. Moreover, the procedure can be applied even after previous Giemsa staining of the preparation, enabling revision of patient smears which were difficult to read after routine Giemsa staining.
本文描述了一种高度灵敏的非放射性DNA原位杂交方法，该方法能够检测并明确鉴定人类和啮齿类疟原虫的各个发育阶段。使用生物素化的物种特异性DNA探针，可在血涂片上对红细胞内的疟原虫进行特异性染色。同样，也能在细胞培养物和石蜡包埋肝脏切片中观察到红细胞外期疟原虫。在血涂片中，杂交程序能让经验丰富的显微镜检查人员在100至400倍放大倍数下快速检测到（低水平的）疟原虫血症并确定疟原虫种类。此外，即使在制备物先前已用吉姆萨染色后，该程序仍可应用，从而能够复查那些在常规吉姆萨染色后难以判读的患者涂片。
Zotero 插件