通过细胞化学杂交在疟原虫中检测到的核糖体DNA序列。

Ribosomal DNA sequences detected in malaria parasites by cytochemical hybridization.

作者信息

Cornelissen A W, Langsley G, Walliker D

出版信息

Biol Cell. 1983;49(1):87-90. doi: 10.1111/j.1768-322x.1984.tb00225.x.

DOI:10.1111/j.1768-322x.1984.tb00225.x

Abstract

A procedure in which fluorochrome-labelled RNA is hybridized in situ to homologous DNA sequences was used to investigate the possible application of this method to the human malaria parasite Plasmodium falciparum. Rhodamine-labelled ribosomal RNA stained the nuclei of the parasites after cytochemical hybridization. This result demonstrates that ribosomal RNA genes can be visualised. It was estimated that the hybridization efficiency was greater than 40%.

摘要

一种使荧光染料标记的RNA原位与同源DNA序列杂交的方法被用于研究该方法在人类疟原虫恶性疟原虫上的可能应用。细胞化学杂交后，罗丹明标记的核糖体RNA对疟原虫的细胞核进行了染色。这一结果表明核糖体RNA基因可以被可视化。据估计，杂交效率大于40%。