内皮祖细胞对犬组织工程骨骨形成影响的实验研究
[An experimental study of endothelial progenitor cells on bone formation in tissue-engineered bone in dogs].
作者信息
Gao Zhen-hua, Bu Ling-xue, Yuan Rong-tao, Feng Yuan-yong, Wang Ling-ling, Li Ning-yi
机构信息
Department of Oral and Maxillofacial Surgery, the Affiliated Hospital of Qingdao University Medical College. Qingdao 266003, Shandong Province, China.
出版信息
Shanghai Kou Qiang Yi Xue. 2011 Aug;20(4):342-6.
PURPOSE
To observe the effects of endothelial progenitor cells (EPCs) on bone formation of tissue engineered bone in dogs.
METHODS
Bone marrow stromal cells (BMSCs) and EPCs were derived from dog bone marrow and cultured in different medium in vitro. They were seeded on demineralized bone matrix (DBM) to build tissue engineered bone, then the construct was implanted into the fasciae of latissimus dorsi muscle, the degree of bone formation was analyzed with imaging and histological methods at different time points. SPSS13.0 software package was used for statistical analysis.
RESULTS
4, 8, 12 weeks after operation, X-ray film showed bone mineral density (BMD) in the EPCs group was significantly higher than the control group, and there was significant difference between the two groups(P<0.05); Histological examination revealed that the degree of bone formation in the EPCs group was higher than the control group, the new bone area and blood vessel area between the two groups were significantly different(P<0.05).
CONCLUSION
EPCs can promote bone formation and accelerate new bone formation in tissue-engineering bone.
目的
观察内皮祖细胞（EPCs）对犬组织工程骨骨形成的影响。
方法
从犬骨髓中分离出骨髓基质细胞（BMSCs）和EPCs，并在体外不同培养基中培养。将它们接种于脱矿骨基质（DBM）上构建组织工程骨，然后将构建体植入背阔肌筋膜，在不同时间点采用影像学和组织学方法分析骨形成程度。使用SPSS13.0软件包进行统计分析。
结果
术后4、8、12周，X线片显示EPCs组骨密度（BMD）明显高于对照组，两组间差异有统计学意义（P<0.05）；组织学检查显示EPCs组骨形成程度高于对照组，两组间新骨面积和血管面积差异有统计学意义（P<0.05）。
结论
EPCs可促进组织工程骨的骨形成并加速新骨形成。
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