Bu Ling-xue, Wang Yan-hui, Li Ning-yi, Gao Zheng-hua, Chen Xin, Jing Heng
Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Medical College, Qingdao University, Qingdao Shandong 266003, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2011 Dec;46(12):747-50.
To construct tissue engineering bone with bone marrow stromal cell (BMSC) sheets of dogs.
BMSC were derived from dog bone marrow and cell sheets were prepared with temperature-responsive dishes after the cells were induced by osteogenesis. Allogeneic dogs decalcification bone matrixes (DBM) were prepared. Sixteen dogs were divided into 4 groups. The MSC cell sheets-rhBMP-2-BMSC-DBM were implanted under the left latissimus dorsi myofascial as the experimental side; while the rhBMP-2-BMSC-DBM were implanted in the right side as the control. Ectopic bone formation in vivo was evaluated by histological examination 4, 8, 12, 16 weeks after operation.
The osteogenesis in the experimental group was better than that in the control group. New bone area in the experimental side was larger than that in the control group, and the difference was significant (P < 0.05). After 16 weeks, lamellar bone was connected into a film in the experimental group. Haversian system and red bone marrow could be seen.
BMSC cell sheets could promote the bone formation of functional tissue-engineered bone.
构建犬骨髓基质细胞（BMSC）片层组织工程骨。
从犬骨髓中获取BMSC，细胞经成骨诱导后用温度响应培养皿制备细胞片层。制备同种异体犬脱钙骨基质（DBM）。将16只犬分为4组。将MSC细胞片层-rhBMP-2-BMSC-DBM植入左侧背阔肌肌筋膜下作为实验侧；而将rhBMP-2-BMSC-DBM植入右侧作为对照。术后4、8、12、16周通过组织学检查评估体内异位骨形成情况。
实验组的成骨情况优于对照组。实验侧新骨面积大于对照组，差异有统计学意义（P < 0.05）。16周后，实验组板层骨连接成膜，可见哈弗斯系统和红骨髓。
BMSC细胞片层可促进功能性组织工程骨的骨形成。
