Li Ning-yi, Chen Li-qiang, Chen Tao, Jin Xiao-ming, Yuan Rong-tao
Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Medical College, College of Stomatology, Qingdao University, Qingdao 266003, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2007 Aug;25(4):408-11.
To study the effect of platelet-rich plasma (PRP) and latissimus dorsi myofascia with blood vessel on vascularization of tissue engineered bone in dogs.
Bone marrow stromal cells (BMSCs) were isolated from iliac bone of dogs. PRP was obtained from the same dog. And demineralized bone matrix (DBM) were prepared from homologuous bone. ABCD 4 areas were divided on the back of dog. PRP/BMSCs/DBM was implanted around the vessels of lattisimus dorsi muscle in the A. PRP/BMSCs/DBM wrapped by superficial fascia in the B. BMSCs/DBM was implanted around vessels of lattisimus dorsi muscle in the C. BMSCs/DBM wrapped by superficial fascia in the D area of the same dog. 4, 8, 12 weeks after implantation, gross specimen and histology examination were made.
Osteogenesis and blood vessel formation results were A>B>C>D area.
The results suggested that the PRP and latissimus dorsi myofascia with blood vessels could promote calcification and vascularization in tissue-engineered bone.
研究富血小板血浆（PRP）和带血管的背阔肌肌筋膜对犬组织工程骨血管化的影响。
从犬髂骨分离骨髓间充质干细胞（BMSCs）。PRP取自同一只犬。脱矿骨基质（DBM）由同种异体骨制备。在犬背部划分ABCD 4个区域。A区将PRP/BMSCs/DBM植入背阔肌血管周围；B区将PRP/BMSCs/DBM用浅筋膜包裹；C区将BMSCs/DBM植入背阔肌血管周围；D区将BMSCs/DBM用同一只犬的浅筋膜包裹。植入后4、8、12周，进行大体标本和组织学检查。
成骨和血管生成结果为A区＞B区＞C区＞D区。
结果表明，PRP和带血管的背阔肌肌筋膜可促进组织工程骨的钙化和血管化。
