Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, Japan.
Plant Cell Rep. 2012 Jan;31(1):103-10. doi: 10.1007/s00299-011-1143-6. Epub 2011 Sep 11.
A single-chain variable fragment antibody (scFv) against plumbagin (PL) accumulated the PL production in the hairy roots of Plumbago zeylanica. Recombinant Agrobacterium rhizogenes (ATCC 15834) containing an scFv gene against PL (PL-scFv) were obtained through triparental mating and transformed into P. zeylanica to induce PL-scFv protein in the hairy roots. Up to 40 μg recombinant PL-scFv were expressed per milligram of soluble protein in transgenic P. zeylanica hairy root cultures. The mean PL content obtained from transgenic hairy roots (12.24 μg/100 mg dry weight) exhibited 2.2 times higher than those obtained from wild-type (5.48 μg/100 mg dry weight). The high correlation between the PL-scFv expression level and PL content of the recombinant plants suggested that the PL biosynthesis pathway had been modulated by the expression of PL-scFv protein in the hairy roots of P. zeylanica.
针对白花丹素（PL）的单链可变片段抗体（scFv）使白花丹的发根中积累了 PL。通过三亲交配获得了含有针对 PL 的 scFv 基因的重组根瘤农杆菌（ATCC 15834）（PL-scFv），并将其转化为白花丹以在发根中诱导 PL-scFv 蛋白。在转基因白花丹发根培养物中，每毫克可溶性蛋白可表达多达 40μg的重组 PL-scFv。从转基因发根中获得的 PL 含量平均值（12.24μg/100mg 干重）比从野生型中获得的（5.48μg/100mg 干重）高 2.2 倍。PL-scFv 表达水平与重组植物 PL 含量之间的高度相关性表明，PL 生物合成途径已通过在白花丹发根中表达 PL-scFv 蛋白进行了调节。
