Jaisi Amit, Panichayupakaranant Pharkphoom
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, 90112, Thailand.
Excellent Research Laboratory, Phytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla, 90112, Thailand.
Biotechnol Lett. 2016 Feb;38(2):351-5. doi: 10.1007/s10529-015-1969-z. Epub 2015 Oct 1.
To evaluate the effects of 12 biotic and abiotic elicitors for increasing the production of plumbagin in Plumbago indica root cultures.
Most elicitors showed minimal effects on the root dry weight, except for 250 mg chitosan l(-1) and 10 mM L-alanine that markedly decreased root biomass by about 40 % compared to the untreated root cultures (5 g l(-1)). Treatments with 100 µM AgNO3 significantly increased intracellular plumbagin production by up to 7.6 mg g(-1) DW that was 4-fold more than the untreated root cultures (1.9 mg g(-1) DW). In contrast, treatments with 150 mg chitosan l(-1), 5 mM L-alanine, and 50 µM 1-naphthol significantly enhanced the extracellular secretion of plumbagin by up to 10.6, 6.9, and 5.7 mg g(-1) DW, respectively, and increased the overall production of plumbagin by up to 12.5, 12.5, and 9.4 mg g(-1) DW, respectively.
Chitosan (150 mg l(-1)), L-alanine (5 mM), and 1-naphthol (50 µM) were the best elicitors to enhance plumbagin production in P. indica root cultures.
评估12种生物和非生物诱导子对增加白花丹根培养物中白花丹素产量的影响。
大多数诱导子对根干重影响极小，除了250 mg壳聚糖l(-1)和10 mM L-丙氨酸，与未处理的根培养物（5 g l(-1)）相比，它们使根生物量显著降低约40%。用100 µM硝酸银处理显著提高了细胞内白花丹素产量，最高可达7.6 mg g(-1)干重，是未处理根培养物（1.9 mg g(-1)干重）的4倍。相比之下，用150 mg壳聚糖l(-1)、5 mM L-丙氨酸和50 µM 1-萘酚处理分别显著提高了白花丹素的细胞外分泌量，最高可达10.6、6.9和5.7 mg g(-1)干重，并分别使白花丹素的总产量提高了12.5、12.5和9.4 mg g(-1)干重。
壳聚糖（150 mg l(-1)）、L-丙氨酸（5 mM）和1-萘酚（50 µM）是提高白花丹根培养物中白花丹素产量的最佳诱导子。
