Department of Botany, Savitribai Phule Pune University, Pune 411007, India.
Department of Microbiology, Savitribai Phule Pune University, Pune 411007, India.
Fitoterapia. 2020 Nov;147:104761. doi: 10.1016/j.fitote.2020.104761. Epub 2020 Oct 16.
Plumbagin is a pharmacologically active naphthoquinone present in the Plumbago zeylanica L. having important medicinal properties. The root of P. zeylanica is rich and primary tissue of the plumbagin biosynthesis and accumulation. The complete biosynthetic pathway of plumbagin in plant is still obscure. The present study attempts to understand the plumbagin biosynthetic pathway with the help of differential transcriptome and metabolome analysis of P. zeylanica leaf and root. The transcriptome data showed co-expression of Aldo-keto reductase (PzAKR), Polyketide cyclase (Pzcyclase) and Cytochrome P450 (PzCYPs) transcripts along with the Polyketide synthase (PzPKS) transcripts. Their higher expression in root as compared to leaf supports their possible involvement in plumbagin biosynthesis. The metabolome data of leaf and root revealed naphthalene derivative isoshinanolone that could be potential precursor of plumbagin. Pathway elucidation and transcriptome data of P. zeylanica, will enable and accelerate research on naphthoquinone biosynthesis in plants.
白花丹素是一种具有重要药用特性的萘醌类化合物，存在于白花丹（Plumbago zeylanica L.）中。该植物的根富含萘醌生物合成和积累的初生组织。植物中白花丹素的完整生物合成途径仍然不清楚。本研究试图通过对白花丹叶和根的差异转录组和代谢组分析来了解白花丹素的生物合成途径。转录组数据显示，醛酮还原酶（PzAKR）、聚酮合酶（PzPKS）、多酮环化酶（Pzcyclase）和细胞色素 P450（PzCYPs）转录物的共表达，以及多酮合酶（PzPKS）转录物的共表达。与叶相比，根中这些转录物的表达水平更高，这支持它们可能参与白花丹素的生物合成。叶和根的代谢组数据显示萘衍生物异山奈酚酮可能是白花丹素的潜在前体。对白花丹（Plumbago zeylanica）的途径阐明和转录组数据将能够并加速植物中萘醌生物合成的研究。
