Department of Biological Engineering, Inha University, 253 Yonghyun-dong, Nam-gu, Incheon, 402-751, Korea.
Bioprocess Biosyst Eng. 2012 Jan;35(1-2):35-41. doi: 10.1007/s00449-011-0600-5. Epub 2011 Sep 11.
Alkaline-oxidative (A/O) pretreatment and enzymatic saccharification were optimized for bioethanol fermentation from water hyacinth by Saccharomyces cerevisiae. Water hyacinth was subjected to A/O pretreatment at various NaOH and H(2)O(2) concentrations and reaction temperatures for the optimization of bioethanol fermentation by S. cerevisiae. The most effective condition for A/O pretreatment was 7% (w/v) NaOH at 100 °C and 2% (w/v) H(2)O(2). The carbohydrate content was analyzed after reaction at various enzyme concentrations and enzyme ratios using Celluclast 1.5 L and Viscozyme L to determine the effective conditions for enzymatic saccharification. After ethanol fermentation using S. cerevisiae KCTC 7928, the concentration of glucose, ethanol and glycerol was analyzed by HPLC using a RI detector. The yield of ethanol in batch fermentation was 0.35 g ethanol/g biomass. Continuous fermentation was carried out at a dilution rate of 0.11 (per h) and the ethanol productivity was 0.77 [g/(l h)].
蒌蒿的碱性氧化（A/O）预处理和酶解糖化优化用于酿酒酵母的生物乙醇发酵。蒌蒿经过 A/O 预处理，采用不同浓度的 NaOH 和 H2O2 以及反应温度，以优化酿酒酵母的生物乙醇发酵。A/O 预处理的最佳条件为 7%（w/v）NaOH 在 100°C 和 2%（w/v）H2O2。使用 Celluclast 1.5 L 和 Viscozyme L 在不同酶浓度和酶比下进行反应后分析碳水化合物含量，以确定酶解糖化的有效条件。使用酿酒酵母 KCTC 7928 进行乙醇发酵后，通过高效液相色谱法（HPLC）使用 RI 检测器分析葡萄糖、乙醇和甘油的浓度。分批发酵的乙醇产率为 0.35 g 乙醇/g 生物质。在稀释率为 0.11（每小时）进行连续发酵，乙醇生产率为 0.77 [g/（l h）]。
