Department of Biotechnology, Pukyong National University, Busan, 608-737, Korea.
Bioprocess Biosyst Eng. 2019 Aug;42(8):1255-1262. doi: 10.1007/s00449-019-02123-8. Epub 2019 Apr 17.
In this study, Ascophyllum nodosum was studied as a biomass for ethanol production. A. nodosum was degraded to monosaccharide by hyper-thermal (HT) acid hydrolysis and enzymatic saccharification and analyzed using response surface methodology (RSM) and the Michaelis-Menten equation. Maximum monosaccharide concentrations of 20.3 g/L glucose and 7.0 g/L mannitol were obtained from HT acid hydrolysis and enzymatic saccharification from 8%(w/v) of A. nodosum. Fermentation was conducted using Pichia stipitis and P. angophorae adapted to high mannitol concentrations. Neither non-adapted P. stipitis and P. angophorae nor adapted P. stipitis could ferment all mannitol in the A. nodosum hydrolysate. Adapted P. angophorae produced the highest ethanol concentration among various yeasts, with ethanol production reaching 13.6 g/L with an ethanol yield (Y) of 0.50. Optimization of HT acid hydrolysis and enzymatic saccharification, in combination with the use of adapted yeast, could enhance overall A. nodosum ethanol fermentation yields.
在这项研究中，我们将泡叶藻用作生产乙醇的生物质。通过超热（HT）酸水解和酶解将泡叶藻降解为单糖，并使用响应面法（RSM）和米氏方程进行分析。从 8%（w/v）的泡叶藻中，通过 HT 酸水解和酶解分别获得了 20.3 g/L 葡萄糖和 7.0 g/L 甘露醇的最大单糖浓度。使用适应高甘露醇浓度的毕赤酵母（Pichia stipitis）和短梗霉（P. angophorae）进行发酵。未经驯化的毕赤酵母和短梗霉均不能发酵泡叶藻水解液中的所有甘露醇，而适应的毕赤酵母则可以。各种酵母中，适应的短梗霉产生的乙醇浓度最高，乙醇产量达到 13.6 g/L，乙醇得率（Y）为 0.50。HT 酸水解和酶解的优化以及适应酵母的使用可以提高泡叶藻的整体乙醇发酵产率。
