Department of Biotechnology, Pukyong National University, Busan, 48513, South Korea.
Bioprocess Biosyst Eng. 2019 Aug;42(8):1367-1374. doi: 10.1007/s00449-019-02136-3. Epub 2019 May 6.
Water hyacinth (Eichhornia crassipes) was used as a feedstock for ethanol production. The optimal hyper-thermal (HT) acid hydrolysis conditions were 8% (w/v) slurry content, 200 mM HSO, at 160 °C for 20 min and enzymatic saccharification for 48 h using an enzyme mixture of 20 units/mL Viscozyme L and Cellic C Tec2. After pretreatment, 48.2 g/L monosaccharides were obtained. Fermentation was conducted with wild and adapted Saccharomyces cerevisiae, Pichia stipitis and Candida lusitaniae. Wild-type S. cerevisiae, P. stipitis, and C. lusitaniae produced 15.3, 19.5 and 22.7 g/L of ethanol, respectively. Adaptive evolution was carried out on 6% (w/v) xylose. S. cerevisiae, P. sipitis and C. lusitaniae adapted to xylose produced 15.3, 21.4 and 23.9 g/L of ethanol with Y of 0.32, 0.44 and 0.49, respectively. These results indicate that water hyacinth has potential as a feed stock for ethanol.
水葫芦被用作生产乙醇的原料。最佳超热（HT）酸水解条件为 8%（w/v）浆料含量、200 mM HSO 在 160°C 下反应 20 分钟，然后使用 20 个单位/mL Viscozyme L 和 Cellic C Tec2 的酶混合物进行 48 小时酶解糖化。预处理后，可获得 48.2 g/L 的单糖。采用野生型和驯化的酿酒酵母、毕赤酵母和葡萄牙假丝酵母进行发酵。野生型酿酒酵母、毕赤酵母和葡萄牙假丝酵母分别产生 15.3、19.5 和 22.7 g/L 的乙醇。对 6%（w/v）木糖进行了适应性进化。适应木糖的酿酒酵母、毕赤酵母和葡萄牙假丝酵母分别产生 15.3、21.4 和 23.9 g/L 的乙醇，Y 值分别为 0.32、0.44 和 0.49。这些结果表明水葫芦作为乙醇生产的原料具有潜力。
