Zhou Qian-na, Xing Li-min, Fu Rong, Wang Hua-quan, Liu Hong, Li Li-juan, Ruan Er-bao, Zhang Tian, Qu Wen, Wu Yu-hong, Song Jia, Guan Jing, Wang Jun, Shao Zong-hong
Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
Zhonghua Yi Xue Za Zhi. 2011 Jun 28;91(24):1687-90.
To explore the effects of CD8(+)CXCR3(+)T cells on autoimmune hemolytic anemia (AIHA).
Twenty-two AIHA patients, including 11 untreated and 11 recovered ones, and 23 normal controls were recruited from July 2010 to November 2010. The percentage of CD8(+)CXCR3(+)/CD8(+)T cells in peripheral blood and the expression of interleukin-10 (IL-10) in CD8(+)CXCR3(+)T cells were detected by flow cytometry. Their correlations with the count of CD3(+)CD4(+)cells and the percentage of CD5(+)CD19(+) in CD19(+) B cells were analyzed. The expression level of CXCR3 mRNA in PBMC was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
The percentage of CD8(+)CXCR3(+)/CD8(+) of untreated AIHA patients was (39.80 ± 19.96)%. And it was lower than that of recovered patients [(58.76 ± 14.22)%, P < 0.05] and normal controls [(59.66 ± 12.62)%, P < 0.01]. The percentage of IL-10(+) T cells in CD8(+)CXCR3(+)T cells of untreated patients was (22.98 ± 14.96)% and it was lower than that of normal controls [(38.15 ± 17.03)%, P < 0.05]. The expression level of CXCR3 mRNA for untreated AIHA patients was (0.51 ± 0.19) and it was lower than that of normal controls (1.67 ± 1.17, P < 0.01). The percentage of CD8(+)CXCR3(+)/CD8(+)T cells had a negative correlation with the count of CD3(+)CD4(+) cells and the percentage of CD5(+)CD19(+)/CD19(+) B cells (r = -0.571, -0.583, both P < 0.05). So did the percentage of IL-10(+) T cells in CD8(+)CXCR3(+)T cells (r = -0.524, -0.523, both P < 0.05).
The decreased count of CD8(+)CXCR3(+)T cells and the lowered level of IL-10 may disturb the immune tolerance and lead to the occurrence of AIHA.
探讨CD8(+)CXCR3(+)T细胞对自身免疫性溶血性贫血（AIHA）的影响。
选取2010年7月至2010年11月收治的22例AIHA患者，其中未治疗患者11例、康复患者11例，以及23名正常对照者。采用流式细胞术检测外周血中CD8(+)CXCR3(+)/CD8(+)T细胞的百分比及CD8(+)CXCR3(+)T细胞中白细胞介素-10（IL-10）的表达。分析其与CD3(+)CD4(+)细胞计数及CD19(+)B细胞中CD5(+)CD19(+)百分比的相关性。采用半定量逆转录-聚合酶链反应（RT-PCR）检测外周血单个核细胞（PBMC）中CXCR3 mRNA的表达水平。
未治疗AIHA患者CD8(+)CXCR3(+)/CD8(+)T细胞百分比为(39.80±19.96)%，低于康复患者[(58.76±14.22)%，P<0.05]及正常对照者[(59.66±12.62)%，P<0.01]。未治疗患者CD8(+)CXCR3(+)T细胞中IL-10(+)T细胞百分比为(22.98±14.96)%，低于正常对照者[(38.15±17.03)%，P<0.05]。未治疗AIHA患者CXCR3 mRNA表达水平为(0.51±0.19)，低于正常对照者(1.67±1.17，P<0.01)。CD8(+)CXCR3(+)/CD8(+)T细胞百分比与CD3(+)CD4(+)细胞计数及CD5(+)CD19(+)/CD19(+)B细胞百分比呈负相关（r=-0.571、-0.583，均P<0.05）。CD8(+)CXCR3(+)T细胞中IL-10(+)T细胞百分比亦如此（r=-0.524、-0.523，均P<0.05）。
CD8(+)CXCR3(+)T细胞数量减少及IL-10水平降低可能干扰免疫耐受，导致AIHA的发生。
