Institute of Microbiology, Academy of Sciences of the Czech Republic v.v.i., Prague, Czech Republic.
J Antibiot (Tokyo). 2011 Nov;64(11):717-22. doi: 10.1038/ja.2011.81. Epub 2011 Sep 14.
A new polyene macrolide family, closely related to the pentaene macrolide antibiotic roflamycoin, was isolated from the both fermentation broth and biomass of Streptomyces durmitorensis wild-type strain MS405. The main compound was identified by NMR and Fourier transform ion cyclotron resonance mass spectrometry as 32,33-didehydroroflamycoin (1; DDHR). Additional four structurally related compounds were determined solely by MS analysis. DDHR induces cell death by apoptosis in various cancer cell lines as demonstrated by DNA fragmentation. Striking feature of DDHR is its internal fluorescence allowing visualization of labeled plasma membranes and internal membrane structures.
一种新的多烯大环内酯家族，与 pentaene 大环内酯抗生素罗氟米酮密切相关，从 Streptomyces durmitorensis 野生型菌株 MS405 的发酵液和生物量中分离得到。主要化合物通过 NMR 和傅里叶变换离子回旋共振质谱鉴定为 32,33-二去氢罗氟米酮（1；DDHR）。另外四个结构相关的化合物仅通过 MS 分析确定。DDHR 通过 DNA 片段化在各种癌细胞系中诱导细胞凋亡。DDHR 的显著特征是其内部荧光，允许标记的质膜和内部膜结构可视化。
