Prates Renato A, Yamada Aécio M, Suzuki Luis C, França Cristiane M, Cai Silvana, Mayer Márcia P A, Ribeiro Adriana C, Ribeiro Martha S
Center for Lasers and Applications, Instituto de Pesquisas Energéticas Nucleares, IPEN-CNEN/SP, Cidade Universitária , São Paulo, SP, Brazil.
Photomed Laser Surg. 2011 Dec;29(12):835-44. doi: 10.1089/pho.2010.2984. Epub 2011 Sep 14.
The aim of this study was to investigate the short-term effects of photodynamic therapy (PDT) in periodontal tissue when it is used as an adjuvant treatment for periodontitis.
PDT has been used as an adjuvant in the combat of local infections, such as periodontitis, and combines a photosensitizer (PS) with a light source to induce reactive oxygen species (ROS) and kill microbial cells.
Fifty healthy male rats were used in this study. Periodontitis was induced by placing a cotton ligature around the upper left second molar in a subgingival position. Posterior maxillas were removed and histologically prepared with hematoxylin & eosin (H&E) staining techniques. PDT was performed with a diode laser (λ=660 nm) with an output power of 100 mW. Methylene blue aqueous solution (100 μM) was used as the PS while control group used phosphate buffered saline (PBS). Collagen organization, inflammatory infiltrate, and bone loss were evaluated. Bacterial samples were collected before and immediately after treatment to determine bacterial reduction.
The experimental group that was treated with PDT presented better periodontal healing, as measured by collagen organization, inflammatory infiltrate, and bone loss. Significant bacterial reduction was achieved following treatment with or without PDT compared to control, with a higher microbial reduction observed in the PDT group.
PDT used as an adjuvant treatment showed effective short-term control of periodontitis infection.
本研究旨在调查光动力疗法（PDT）作为牙周炎辅助治疗方法时对牙周组织的短期影响。
PDT已被用作对抗局部感染（如牙周炎）的辅助手段，它将光敏剂（PS）与光源结合以产生活性氧（ROS）并杀死微生物细胞。
本研究使用了50只健康雄性大鼠。通过在左上第二磨牙龈下位置放置棉线结扎来诱导牙周炎。切除上颌后部并采用苏木精和伊红（H&E）染色技术进行组织学制备。使用输出功率为100 mW的二极管激光（λ=660 nm）进行PDT。亚甲蓝水溶液（100 μM）用作PS，而对照组使用磷酸盐缓冲盐水（PBS）。评估胶原组织、炎性浸润和骨质流失情况。在治疗前和治疗后立即采集细菌样本以确定细菌减少情况。
通过胶原组织、炎性浸润和骨质流失评估，接受PDT治疗的实验组牙周愈合情况更好。与对照组相比，无论是否进行PDT治疗，治疗后细菌均显著减少，且PDT组的微生物减少率更高。
PDT作为辅助治疗手段显示出对牙周炎感染有效的短期控制效果。
