State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
Insect Mol Biol. 2011 Dec;20(6):745-62. doi: 10.1111/j.1365-2583.2011.01104.x. Epub 2011 Sep 15.
The brown planthopper, Nilaparvata lugens, is a serious pest threatening rice production across the world. To identify the main features of the gene expression and the key components of the midgut of N. lugens responsible for nutrition, xenobiotic metabolism and the immune response, we used pyrosequencing to sample the transcriptome. More than 190,000 clean sequences were generated, which led to about 30,000 unique sequences. Sequence analysis indicated that genes with abundant transcripts in the midgut of N. lugens were mainly sugar hydrolyases and transporters, proteases and detoxification-related proteins. Based on the sequence information, we cloned the candidate sucrase gene; this enzyme is likely to interact with the perimicrovillar membrane through its highly hydrophobic C-terminal region. Many proteases were identified, which supported the hypothesis that N. lugens uses the proteolysis system for digestion. Scores of detoxification genes were newly identified, including cytochrome P450s, glutathione S-transferases, caroxylesterases. A wealth of new transcripts possibly participating in the immune response were described as well. The gene encoding a peptidoglycan recognition protein was cloned. Unlike in Acyrthosiphon pisum, the immunodeficiency pathway may be present in N. lugens. This is the first global analysis of midgut transcriptome from N. lugens.
褐飞虱，Nilaparvata lugens，是一种严重威胁全球水稻生产的害虫。为了鉴定与营养、外来化合物代谢和免疫反应有关的 N. lugens 中基因表达的主要特征和中肠的关键成分，我们使用焦磷酸测序对转录组进行了采样。生成了超过 190,000 个清洁序列，导致约 30,000 个独特序列。序列分析表明，在 N. lugens 中肠中丰度转录的基因主要是糖水解酶和转运蛋白、蛋白酶和解毒相关蛋白。根据序列信息，我们克隆了候选蔗糖酶基因；该酶可能通过其高度疏水性的 C 末端区域与周质膜相互作用。鉴定了许多蛋白酶，支持了 N. lugens 利用蛋白水解系统进行消化的假说。新鉴定了大量解毒基因，包括细胞色素 P450s、谷胱甘肽 S-转移酶、羧酸酯酶。还描述了大量可能参与免疫反应的新转录本。编码肽聚糖识别蛋白的基因被克隆。与 Acyrthosiphon pisum 不同，N. lugens 中可能存在免疫缺陷途径。这是首次对 N. lugens 中肠转录组进行的全球分析。
