RNA干扰（RNAi）在昆虫中的新兴应用策略。
Emerging strategies for RNA interference (RNAi) applications in insects.
作者信息
Nandety Raja Sekhar, Kuo Yen-Wen, Nouri Shahideh, Falk Bryce W
机构信息
a Department of Plant Pathology ; University of California ; Davis , CA USA.
出版信息
Bioengineered. 2015;6(1):8-19. doi: 10.4161/21655979.2014.979701. Epub 2014 Dec 31.
RNA interference (RNAi) in insects is a gene regulatory process that also plays a vital role in the maintenance and in the regulation of host defenses against invading viruses. Small RNAs determine the specificity of the RNAi through precise recognition of their targets. These small RNAs in insects comprise small interfering RNAs (siRNAs), micro RNAs (miRNAs) and Piwi interacting RNAs (piRNAs) of various lengths. In this review, we have explored different forms of the RNAi inducers that are presently in use, and their applications for an effective and efficient fundamental and practical RNAi research with insects. Further, we reviewed trends in next generation sequencing (NGS) technologies and their importance for insect RNAi, including the identification of novel insect targets as well as insect viruses. Here we also describe a rapidly emerging trend of using plant viruses to deliver the RNAi inducer molecules into insects for an efficient RNAi response.
昆虫中的RNA干扰（RNAi）是一种基因调控过程，在维持和调节宿主对入侵病毒的防御中也起着至关重要的作用。小RNA通过精确识别其靶标来决定RNAi的特异性。昆虫中的这些小RNA包括各种长度的小干扰RNA（siRNA）、微小RNA（miRNA）和Piwi相互作用RNA（piRNA）。在本综述中，我们探讨了目前正在使用的RNAi诱导剂的不同形式，以及它们在昆虫有效且高效的基础和实际RNAi研究中的应用。此外，我们回顾了下一代测序（NGS）技术的趋势及其对昆虫RNAi的重要性，包括新型昆虫靶标以及昆虫病毒的鉴定。在这里，我们还描述了一种迅速兴起的趋势，即利用植物病毒将RNAi诱导剂分子递送至昆虫体内以实现高效的RNAi反应。
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