一种用于检测不对称分布 RNA 的遗传体内系统。
A genetic in vivo system to detect asymmetrically distributed RNA.
机构信息
Institute of Genetics, University of Cologne, Zülpicher Strasse 47, Cologne 50674, Germany.
出版信息
EMBO Rep. 2011 Oct 28;12(11):1167-74. doi: 10.1038/embor.2011.178.
Abstract
Many RNAs show polarized or otherwise non-random subcellular distributions. To create a method for genome-wide genetic screens for RNAs with asymmetric subcellular distributions, we have combined methods for gene tagging and live imaging of messenger RNA (mRNA). A pilot screen in a highly polarized, differentiated cell in the Drosophila larva, the branched terminal cell of the tracheal system, demonstrates the feasibility of the method for identifying new asymmetrically localized mRNAs in vivo.
摘要
许多 RNA 显示出极化或其他非随机的亚细胞分布。为了创建一种用于全基因组遗传筛选具有不对称亚细胞分布的 RNA 的方法，我们结合了基因标记和信使 RNA（mRNA）的活体成像方法。在果蝇幼虫中一个高度极化分化的细胞——气管系统的分支末端细胞中的一个初步筛选实验证明了该方法在体内鉴定新的不对称定位 mRNA 的可行性。
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