RNA定位的基因组分析。
Genomic analysis of RNA localization.
作者信息
Taliaferro J Matthew, Wang Eric T, Burge Christopher B
机构信息
a Department of Biology; Massachusetts Institute of Technology ; Cambridge , MA USA.
出版信息
RNA Biol. 2014;11(8):1040-50. doi: 10.4161/rna.32146.
The localization of mRNAs to specific subcellular sites is widespread, allowing cells to spatially restrict and regulate protein production, and playing important roles in development and cellular physiology. This process has been studied in mechanistic detail for several RNAs. However, the generality or specificity of RNA localization systems and mechanisms that impact the many thousands of localized mRNAs has been difficult to assess. In this review, we discuss the current state of the field in determining which RNAs localize, which RNA sequences mediate localization, the protein factors involved, and the biological implications of localization. For each question, we examine prominent systems and techniques that are used to study individual messages, highlight recent genome-wide studies of RNA localization, and discuss the potential for adapting other high-throughput approaches to the study of localization.
mRNA定位于特定亚细胞位点的现象广泛存在，使细胞能够在空间上限制和调节蛋白质的产生，并在发育和细胞生理学中发挥重要作用。这一过程已针对多种RNA进行了详细的机制研究。然而，影响数千种定位mRNA的RNA定位系统和机制的普遍性或特异性一直难以评估。在本综述中，我们讨论了该领域在确定哪些RNA定位、哪些RNA序列介导定位、涉及的蛋白质因子以及定位的生物学意义方面的现状。对于每个问题，我们研究了用于研究单个信息的突出系统和技术，强调了最近全基因组范围的RNA定位研究，并讨论了采用其他高通量方法进行定位研究的潜力。
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