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系统性成像揭示了果蝇发育过程中mRNA的特征和定位变化。

Systematic imaging reveals features and changing localization of mRNAs in Drosophila development.

作者信息

Jambor Helena, Surendranath Vineeth, Kalinka Alex T, Mejstrik Pavel, Saalfeld Stephan, Tomancak Pavel

机构信息

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

出版信息

Elife. 2015 Apr 2;4:e05003. doi: 10.7554/eLife.05003.

DOI:10.7554/eLife.05003
PMID:25838129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4384636/
Abstract

mRNA localization is critical for eukaryotic cells and affects numerous transcripts, yet how cells regulate distribution of many mRNAs to their subcellular destinations is still unknown. We combined transcriptomics and systematic imaging to determine the tissue-specific expression and subcellular distribution of 5862 mRNAs during Drosophila oogenesis. mRNA localization is widespread in the ovary and detectable in all of its cell types-the somatic epithelial, the nurse cells, and the oocyte. Genes defined by a common RNA localization share distinct gene features and differ in expression level, 3'UTR length and sequence conservation from unlocalized mRNAs. Comparison of mRNA localizations in different contexts revealed that localization of individual mRNAs changes over time in the oocyte and between ovarian and embryonic cell types. This genome scale image-based resource (Dresden Ovary Table, DOT, http://tomancak-srv1.mpi-cbg.de/DOT/main.html) enables the transition from mechanistic dissection of singular mRNA localization events towards global understanding of how mRNAs transcribed in the nucleus distribute in cells.

摘要

mRNA定位对真核细胞至关重要，且影响众多转录本，但细胞如何调控众多mRNA向其亚细胞目的地的分布仍不清楚。我们结合转录组学和系统成像技术，以确定果蝇卵子发生过程中5862种mRNA的组织特异性表达和亚细胞分布。mRNA定位在卵巢中广泛存在，并且在其所有细胞类型——体细胞上皮细胞、滋养细胞和卵母细胞中均可检测到。由共同的RNA定位所定义的基因具有独特的基因特征，并且在表达水平、3'UTR长度以及与未定位mRNA的序列保守性方面存在差异。对不同背景下mRNA定位的比较表明，单个mRNA的定位在卵母细胞中以及在卵巢和胚胎细胞类型之间会随时间发生变化。这种基于全基因组规模图像的资源（德累斯顿卵巢表，DOT，http://tomancak-srv1.mpi-cbg.de/DOT/main.html）能够实现从对单个mRNA定位事件的机制剖析向全面理解细胞核中转录的mRNA如何在细胞中分布的转变。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e77e/4384636/43b3d46cecdb/elife05003fs010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e77e/4384636/7a6912398181/elife05003f002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e77e/4384636/43b3d46cecdb/elife05003fs010.jpg

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