Ramírez Manuel, Prieto Isabel, Banegas Inmaculada, Segarra Ana B, Alba Francisco
Unit of Physiology, Department of Health Sciences, University of Jaén, Jaén, Spain.
Methods Mol Biol. 2011;789:287-94. doi: 10.1007/978-1-61779-310-3_18.
The control of neuropeptide function is partially accomplished by aminopeptidases (neuropeptidases), which are the most abundant proteolytic enzymes in brain. Their analysis represents an important and quick tool to reflect the functional status of their endogenous substrates. Here, we describe an improved fluorometric method for the determination of neuropeptidase activities based on the fluorescence produced by β-naphthylamine when released from the artificial substrates aminoacyl-β-naphthylamides (arylamides) under the hydrolytic action of these enzymes.
神经肽功能的控制部分是由氨肽酶（神经肽酶）完成的，氨肽酶是大脑中含量最丰富的蛋白水解酶。对它们的分析是反映其内源性底物功能状态的一种重要且快速的工具。在此，我们描述了一种改进的荧光测定法，用于基于在这些酶的水解作用下从人工底物氨酰基-β-萘胺（芳基酰胺）释放出的β-萘胺产生的荧光来测定神经肽酶活性。
