Sakai Y, Nagase H, Ose Y, Kito H, Sato T, Kawai M, Mizuno M
Gifu Prefectural Institute of Public Health, Japan.
Mutat Res. 1990 Jun;244(2):129-34. doi: 10.1016/0165-7992(90)90061-n.
The inhibitory effects of peony root extract on the mutagenicity of benzo[a]pyrene (B [a]p) have been investigated in the Salmonella typhimurium reversion test. Four kinds of experiments were performed: direct chemical reaction (1) between peony root extract and B [a]p, and (2) between peony root extract and active metabolite(s) of B [a]p, (3) inhibition of metabolic processes of B[a]p with S9 mix, and (4) inhibition of activation on mutagenicity. Peony root extract interfered with the action of enzymes in the S9 mix, and inactivated the activity of B[a]p metabolites. The bio-antimutagenic effect was assayed by reversion in Salmonella typhimurium TA98 and TA100.
在鼠伤寒沙门氏菌回复突变试验中，研究了牡丹根提取物对苯并[a]芘（B[a]p）致突变性的抑制作用。进行了四种实验：（1）牡丹根提取物与B[a]p之间的直接化学反应；（2）牡丹根提取物与B[a]p的活性代谢物之间的直接化学反应；（3）用S9混合物抑制B[a]p的代谢过程；（4）抑制致突变性的激活。牡丹根提取物干扰了S9混合物中酶的作用，并使B[a]p代谢物的活性失活。通过鼠伤寒沙门氏菌TA98和TA100的回复突变来测定生物抗突变作用。
