Niikawa M, Hayashi H, Sato T, Nagase H, Kito H
Department of Public Health, Gifu Pharmaceutical University, Japan.
Mutat Res. 1993 Sep;319(1):1-9. doi: 10.1016/0165-1218(93)90025-9.
Methanol and hot-water extracts of glossy privet (Ligustrum lucidum Ait.) inhibited the mutagenic activity of benzo[a]pyrene in Salmonella typhimurium TA98 with S9 mix. The methanol extract was fractionated with ether and n-hexane. As the active components, oleanolic and ursolic acids were isolated, which were soluble in ether and insoluble in n-hexane. The hot-water extract was fractionated to water, 60% and 100% methanol fractions. Nuezhenide was isolated from the 60% methanol fraction as the active component.
女贞（Ligustrum lucidum Ait.）的甲醇提取物和热水提取物在S9混合存在的情况下，抑制了苯并[a]芘在鼠伤寒沙门氏菌TA98中的诱变活性。甲醇提取物用乙醚和正己烷进行分级分离。作为活性成分，分离出了齐墩果酸和熊果酸，它们可溶于乙醚而不溶于正己烷。热水提取物被分级分离为水相、60%甲醇相和100%甲醇相。从60%甲醇相中分离出了女贞苷作为活性成分。
