Division of Cardiology, Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Am J Physiol Heart Circ Physiol. 2011 Dec;301(6):H2198-206. doi: 10.1152/ajpheart.00781.2011. Epub 2011 Sep 16.
Nearly 40 years ago, the Sagawa laboratory spawned a renaissance in the use of instantaneous ventricular pressure-volume (P-V) relations to assess cardiac function. Since then, this analysis has taken hold as the most comprehensive way to quantify ventricular chamber function and energetics and cardiovascular interactions. First studied in large mammalian hearts and later in humans employing a catheter-based method, P-V analysis was translated to small rodents in the late 1990s by the Kass laboratory. Over the past decade, this approach has become a gold standard for comprehensive examination of in vivo cardiac function in mice, facilitating a new era of molecular cardiac physiology. The catheter-based method remains the most widely used approach in mice. In this brief review, we discuss this instrumentation, the theory behind its use, and how volume signals are calibrated and discuss elements of P-V analysis. The goal is to provide a convenient summary of earlier investigations and insights for users whose primary interests lie in genetic/molecular studies rather than in biomedical engineering.
大约 40 年前，Sagawa 实验室开创了瞬时心室压力-容积（P-V）关系在评估心功能方面应用的复兴。从那时起，这种分析方法已经成为量化心室腔功能和能量学以及心血管相互作用的最全面方法。P-V 分析首先在大型哺乳动物心脏中进行研究，后来在人类中采用基于导管的方法进行研究，Kass 实验室在 20 世纪 90 年代末将其转化为小型啮齿动物。在过去的十年中，这种方法已成为在小鼠体内全面检查心功能的金标准，开创了分子心脏生理学的新时代。基于导管的方法仍然是在小鼠中最广泛使用的方法。在这篇简短的综述中，我们将讨论这种仪器、其使用背后的理论，以及如何校准体积信号，并讨论 P-V 分析的要素。目的是为那些主要兴趣在于遗传/分子研究而不是生物医学工程的用户提供一个方便的早期研究和见解的摘要。
