Abraham Dennis, Mao Lan
Department of Medicine, Duke University Medical Center;
Department of Medicine, Duke University Medical Center.
J Vis Exp. 2015 Sep 17(103):52942. doi: 10.3791/52942.
Cardiac pressure-volume loop analysis is the "gold-standard" in the assessment of load-dependent and load-independent measures of ventricular systolic and diastolic function. Measures of ventricular contractility and compliance are obtained through examination of cardiac response to changes in afterload and preload. These techniques were originally developed nearly three decades ago to measure cardiac function in large mammals and humans. The application of these analyses to small mammals, such as mice, has been accomplished through the optimization of microsurgical techniques and creation of conductance catheters. Conductance catheters allow for estimation of the blood pool by exploiting the relationship between electrical conductance and volume. When properly performed, these techniques allow for testing of cardiac function in genetic mutant mouse models or in drug treatment studies. The accuracy and precision of these studies are dependent on careful attention to the calibration of instruments, systematic conduct of hemodynamic measurements and data analyses. We will review the methods of conducting pressure-volume loop experiments using a conductance catheter in mice.
心脏压力-容积环分析是评估心室收缩和舒张功能的负荷依赖性及负荷非依赖性指标的“金标准”。通过检查心脏对后负荷和前负荷变化的反应来获得心室收缩性和顺应性的指标。这些技术最初是在近三十年前开发的，用于测量大型哺乳动物和人类的心脏功能。通过优化显微手术技术和制造电导导管，已将这些分析应用于小鼠等小型哺乳动物。电导导管利用电导与容积之间的关系来估计血池。如果操作得当，这些技术可用于在基因敲除小鼠模型或药物治疗研究中测试心脏功能。这些研究的准确性和精确性取决于对仪器校准的仔细关注、血流动力学测量和数据分析的系统进行。我们将回顾使用电导导管在小鼠中进行压力-容积环实验的方法。
