Dickey Jennifer S, Zemp Franz J, Martin Olga A, Kovalchuk Olga
Laboratory of Biochemistry, Division of Therapeutic Proteins, CDER, FDA, Bethesda, MD 20892, USA.
Radiat Environ Biophys. 2011 Nov;50(4):491-9. doi: 10.1007/s00411-011-0386-5. Epub 2011 Sep 18.
This review focuses on a number of recent studies that have examined changes in microRNA (miRNA) expression profiles in response to ionizing radiation and other forms of oxidative stress. In both murine and human cells and tissues, a number of miRNAs display significant alterations in expression levels in response to both direct and indirect radiation exposure. In terms of direct irradiation, or exposure to agents that induce oxidative stress, miRNA array analyses indicate that a number of miRNAs are up- and down-regulated and, in particular, the let-7 family of miRNAs may well be critical in the cellular response to oxidative stress. In bystander cells that are not directly irradiated, but close to, or share media with directly irradiated cells or tissues, the miRNA expression profiles are also altered, but are somewhat distinct from the directly irradiated cells. Based on the results of these numerous studies, as well as our own data presented here, we conclude that miRNA regulation is a critical step in the cellular response to radiation and oxidative stress and that future studies should elucidate the mechanisms through which this altered regulation affects cell metabolism.
本综述聚焦于近期的一些研究，这些研究探讨了微小RNA（miRNA）表达谱在响应电离辐射和其他形式氧化应激时的变化。在小鼠和人类细胞及组织中，许多miRNA在直接和间接辐射暴露后，其表达水平均显示出显著改变。就直接照射或暴露于诱导氧化应激的试剂而言，miRNA阵列分析表明，许多miRNA的表达上调或下调，特别是let-7家族的miRNA很可能在细胞对氧化应激的反应中起关键作用。在未直接受照射，但靠近直接受照射的细胞或组织，或与直接受照射的细胞或组织共用培养基的旁观者细胞中，miRNA表达谱也会发生改变，但与直接受照射的细胞有所不同。基于这些众多研究的结果以及我们在此展示的自身数据，我们得出结论，miRNA调控是细胞对辐射和氧化应激反应中的关键步骤，未来的研究应阐明这种改变的调控影响细胞代谢的机制。
