Guo Chang-qing, Ji Bo, Chen You-nan, Zhong Ding-wen, Jin Yan, Liu Qing-guo, Guo Meng-wei, Zhang Yi, Liu Nai-gang, Chen Zhan-lu
College of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China.
Zhongguo Gu Shang. 2011 Aug;24(8):656-8.
To study the analgesia mechanism of needle-knife lysis in spinal cord and other parts of central nervous system by comparing the changes of Leu-Enkephalin (L-ENK) content in different parts of centrium of rats undergone needle-knife lysis and electro-acupuncture respectively.
Sixty healthy SD rats were randomly devided into normal control group, model group, needle-knife lysis (NKL) group and electro-acupuncture (EA) group. 4% papain solution mixed with 0.3 mol/L cysteine solutin in the ratio of 1:1, paused for 0.5 h,injected the mixture, 20 microl each time,into the left knee joint cavities of rats in model, NKL, EA groups at the 1st, 4th, 7th day. After 4 weeks in NKL group and EA group were treated with needle-knife lysis and electro-acupuncture, respectively. Three weeks after treatment, samples of spinal cord of the swollen part of rat waists and rat brains were taken from and the content of L-ENK of medulla oblongata, midbrain, pituitary gland, and hippocampus were measured.
L-ENK content of model group increased higher than that of normal control group in spinal cord, hippocampus and midbrain (P < 0.01); there were no significant difference between normal control group and modle group on L-ENK in medulla oblongata and thalamus (P > 0.05). After intervening of NKL or EA, L-ENK content of NKL group increased higher in hippocampus than that of model group and EC group (P < 0.05); but L-ENK content of NKL group in midbrain was lower than that of model group (P < 0.05).
Needle-knife lysis has characteristic of regulation for the L-ENK content in different parts of central nervous system of rats with knee osteoarthritis, and analgesic effect of needle-knife was possibly related with regulation of center L-ENK.
通过比较分别接受针刀松解和电针治疗的大鼠中枢不同部位亮氨酸脑啡肽（L-ENK）含量的变化，研究针刀松解术在脊髓等中枢神经系统部位的镇痛机制。
将60只健康SD大鼠随机分为正常对照组、模型组、针刀松解（NKL）组和电针（EA）组。将4%木瓜蛋白酶溶液与0.3mol/L半胱氨酸溶液按1:1比例混合，静置0.5小时，每次20微升，在第1、4、7天注射到模型组、NKL组、EA组大鼠的左膝关节腔内。4周后，NKL组和EA组分别进行针刀松解和电针治疗。治疗3周后，取大鼠腰部肿胀部位脊髓及大鼠脑样本，测定延髓、中脑、垂体和海马的L-ENK含量。
模型组脊髓、海马和中脑的L-ENK含量高于正常对照组（P<0.01）；正常对照组与模型组延髓和丘脑的L-ENK含量无显著差异（P>0.05）。NKL或EA干预后，NKL组海马的L-ENK含量高于模型组和EA组（P<0.05）；但NKL组中脑的L-ENK含量低于模型组（P<0.05）。
针刀松解对膝骨关节炎大鼠中枢神经系统不同部位的L-ENK含量具有调节作用，针刀的镇痛作用可能与中枢L-ENK的调节有关。
