高水平的CD44表达可区分未接触抗原的B细胞和已接触抗原的B细胞。
High levels of CD44 expression distinguish virgin from antigen-primed B cells.
作者信息
Camp R L, Kraus T A, Birkeland M L, Puré E
机构信息
Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York 10021.
出版信息
J Exp Med. 1991 Mar 1;173(3):763-6. doi: 10.1084/jem.173.3.763.
Abstract
The in vitro polyclonal stimulation of B cells through their surface immunoglobulin (Ig) induces substantial increases in CD44 protein levels within 24 hours, whereas other stimuli (e.g., lipopolysaccharide, phorbol 12,13 dibutyrate, and interleukin 4) fail to significantly upregulate CD44. The marked increase in CD44 protein expression on anti-Ig-treated B lymphocytes correlates with an increase in CD44-specific mRNA. Cell sorting experiments with B cells isolated from trinitrophenyl-keyhole limpet hemocyanin-immunized mice demonstrate that both short-term antigen-specific, IgG-secreting cells and long-term antigen-primed B cells are exclusively CD44high. We speculate that the rapid and sustained increase in CD44 expression mediated by surface Ig stimulation may alter the homing properties of antigen-primed B cells.
摘要
通过B细胞表面免疫球蛋白（Ig）对其进行体外多克隆刺激可在24小时内使CD44蛋白水平大幅增加，而其他刺激（如脂多糖、佛波酯12,13 -二丁酸酯和白细胞介素4）则无法显著上调CD44。抗Ig处理的B淋巴细胞上CD44蛋白表达的显著增加与CD44特异性mRNA的增加相关。对从三硝基苯基-钥孔戚血蓝蛋白免疫小鼠中分离出的B细胞进行细胞分选实验表明，短期抗原特异性IgG分泌细胞和长期抗原致敏B细胞均为CD44高表达。我们推测，表面Ig刺激介导的CD44表达的快速和持续增加可能会改变抗原致敏B细胞的归巢特性。
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