Laboratory of Neurophysiology, Department of Physiology, Medical Faculty, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia.
Molecules. 2011 Sep 19;16(9):8062-75. doi: 10.3390/molecules16098062.
The purpose of this study was to investigate the effects of treatment with water, n-butanol and ether extracts of Hypercom perforatum L. on epileptogenesis in rabbits. Animals from the control group received solvent-ethanol, and the kindling model of epilepsy was used. Epileptic focus was induced in Chinchilla rabbits by stimulation of the hippocampus. The following parameters were determined: the minimum current strength necessary to induce after-discharge (AD) - discharges appearing after cessation of stimulation; AD duration; the number of stimulations necessary to induce spontaneous kindling; and the latency time for the development of full kindling. The results obtained indicate that epileptogenesis is influenced by Hypericum perforatum L. extract treatment. Animals treated with an ether extract of Hypericum perforatum L. required significantly weaker minimum current strengths for the development of epileptogenic focus, and displayed longer AD times, while the number of electro-stimulations necessary for full kindling was less. In contrast, animals treated with water and n-butanol extracts required increased electro-stimulations for the development of epileptic discharge, and displayed shortened AD durations versus controls.
本研究旨在探讨贯叶金丝桃的水提物、正丁醇提物和乙醚提物对兔癫痫发生的影响。对照组动物给予溶剂-乙醇，并采用癫痫点燃模型。通过刺激海马诱导奇努克兔的癫痫灶。测定了以下参数：诱发后放电（AD）所需的最小电流强度-刺激停止后出现的放电；AD 持续时间；诱发自发性点燃所需的刺激次数；以及完全点燃发展的潜伏期。结果表明，贯叶金丝桃提取物的处理影响癫痫发生。用贯叶金丝桃乙醚提取物处理的动物，发展致痫灶所需的最小电流强度明显较弱，AD 时间较长，而完全点燃所需的电刺激次数较少。相比之下，用贯叶金丝桃水提物和正丁醇提取物处理的动物，发展癫痫放电所需的电刺激增加，AD 持续时间缩短，与对照组相比。
