Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21 000 Novi Sad, Serbia.
Molecules. 2013 Sep 25;18(10):11733-50. doi: 10.3390/molecules181011733.
The study shows the influence of the origin of plant material and biological source on the in vitro antioxidant (neutralization of DPPH and OH radical, nitric oxide, and inhibition of lipid peroxidation) and anticholinesterase activity of chemically characterized and quantified ethanol extracts of ten St. John's wort samples. The investigated samples were: five Hypericum perforatum species representatives collected at different localities, one commercial sample of Hyperici herba purchased at a local market and four Hypericum species autochtonous to the Balkan Peninsula (H. maculatum subsp. immaculatum, H. olympicum, H. richeri subsp. grisebachii and H. barbatum). All the examined extracts exhibited notable antioxidant potential, but in most of the cases indigenous Hypericum species expressed stronger effects compared to the original source of the drug, H. perforatum. The changes in the content of phenolic compounds, especially flavonoids, hyperforin and hypericin, related to the source of the drug affected the investigated activities. Since all of the investigated species have shown prominent inhibition of acetylcholinesterase in vitro activity, they could be further investigated as potential substances in preventing of Alzheimer's disease.
研究表明，植物材料的起源和生物来源对十种贯叶连翘样品的体外抗氧化（DPPH 和 OH 自由基中和、一氧化氮抑制和脂质过氧化抑制）和乙酰胆碱酯酶活性的化学特征和定量乙醇提取物有影响。研究的样品包括：五个不同产地的贯叶连翘种代表、一个在当地市场购买的贯叶连翘商品样本以及四个巴尔干半岛土生土长的贯叶连翘种（贯叶连翘亚种。immaculatum、H. olympicum、H. richeri subsp. grisebachii 和 H. barbatum）。所有被检查的提取物都表现出显著的抗氧化潜力，但在大多数情况下，与药物的原始来源贯叶连翘相比，土生土长的贯叶连翘种表现出更强的效果。与药物来源相关的酚类化合物（尤其是类黄酮、金丝桃素和贯叶连翘素）含量的变化影响了所研究的活性。由于所有被研究的物种都表现出体外显著抑制乙酰胆碱酯酶的活性，因此它们可以进一步作为预防阿尔茨海默病的潜在物质进行研究。
