牵引试验与多光子成像相结合以量化皮肤生物力学

Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics.

作者信息

Bancelin Stéphane, Lynch Barbara, Bonod-Bidaud Christelle, Dokládal Petr, Ruggiero Florence, Allain Jean-Marc, Schanne-Klein Marie-Claire

机构信息

Laboratoire d'Optique et Biosciences (LOB), Ecole Polytechnique, CNRS, INSERM, Université Paris-Saclay, Palaiseau, France.

Institut Interdisciplinaire de Neurosciences, CNRS, Université de Bordeaux, Bordeaux, France.

出版信息

Methods Mol Biol. 2019;1944:145-155. doi: 10.1007/978-1-4939-9095-5_11.

DOI:10.1007/978-1-4939-9095-5_11

PMID:30840241

Abstract

An important issue in tissue biomechanics is to decipher the relationship between the mechanical behavior at macroscopic scale and the organization of the collagen fiber network at microscopic scale. Here, we present a protocol to combine traction assays with multiphoton microscopy in ex vivo murine skin. This multiscale approach provides simultaneously the stress/stretch response of a skin biopsy and the collagen reorganization in the dermis by use of second harmonic generation (SHG) signals and appropriate image processing.

摘要

组织生物力学中的一个重要问题是解读宏观尺度下的力学行为与微观尺度下胶原纤维网络组织之间的关系。在此，我们提出一种将体外小鼠皮肤的牵引试验与多光子显微镜相结合的方案。这种多尺度方法通过利用二次谐波产生（SHG）信号和适当的图像处理，同时提供皮肤活检的应力/拉伸反应以及真皮中的胶原重组情况。