Tian Na, Tang Yuwei, Tian Dongming, Liu Zhonghua, Liu Shuoqian
Hunan Collaborative Innovation for Utilization of Botanical Functional Ingredients, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, College of Horticulture and Hardening, Hunan Agricultural University, Changsha, 410128, China.
Department of Tea Science, College of Horticulture and Hardening, Hunan Agricultural University, Changsha, 410128, China.
Biomed Chromatogr. 2017 Mar;31(3). doi: 10.1002/bmc.3824. Epub 2016 Sep 18.
Dihydroartemisinic acid (DHAA) is the direct precursor to artemisinin, an effective anti-malaria compound from Artemisia annua L. (A. annua), and it can be transformed to artemisinin without the catalysis of enzyme. A rapid and sensitive analysis of DHAA in A. annua is needed to screen excellent plant resources aimed to improve artemisinin production. In order to develop a rapid and sensitive determination method for DHAA in plant, the extraction and analysis conditions were extensively investigated in the present work. As a result, extraction of powdered A. annua leaves at 55°C for 50 min with chloroform resulted in the highest yield of DHAA, with a recovery of >98%. The precision of this gas chromatographic procedure ranged from 1.22 to 2.94% for intra-day and from 1.69 to 4.31% for inter-day, respectively. The accuracy was 99.55-103.02% for intra-day and 98.86-99.98% for inter-day, respectively. The measured LOQ and LOD values of the proposed method reached 5.00 and 2.00 μg/mL, respectively. Validation indicated the method was robust, quick, sensitive and adequate for DHAA analysis.
二氢青蒿酸（DHAA）是青蒿素的直接前体，青蒿素是一种从黄花蒿中提取的有效的抗疟化合物，并且它可以在无酶催化的情况下转化为青蒿素。为了筛选出能提高青蒿素产量的优良植物资源，需要对黄花蒿中的二氢青蒿酸进行快速灵敏的分析。为了建立一种快速灵敏的植物中二氢青蒿酸的测定方法，本研究对提取和分析条件进行了广泛的研究。结果表明，用氯仿在55℃下提取黄花蒿粉末叶片50分钟，二氢青蒿酸的产量最高，回收率>98%。该气相色谱法的日内精密度在1.22%至2.94%之间，日间精密度在1.69%至4.31%之间。日内准确度分别为99.55 - 103.02%，日间准确度分别为98.86 - 99.98%。该方法测得的定量限（LOQ）和检测限（LOD）分别达到5.00和2.00μg/mL。验证表明该方法稳健、快速、灵敏，适用于二氢青蒿酸的分析。
