Liu Shuoqian, Ferreira Jorge Freire da Silva, Tian Dongming, Tang Yuwei, Liu Liping, Yang Wei, Liu Zhonghua, Tian Na
Department of Tea Science, College of Horticulture and Hardening, Hunan Agricultural University.
National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals.
Chem Pharm Bull (Tokyo). 2018 Mar 1;66(3):319-326. doi: 10.1248/cpb.c17-00927. Epub 2017 Dec 28.
In order to make full use of artemisinin production waste and thus to reduce the production cost of artemisinin, we developed an efficient and scalable method to isolate high-purity dihydroartemisinic acid from artemisinin production waste by combining anion-exchange resin with silica-gel column chromatography. The adsorption and desorption characteristics of dihydroartemisinic acid on 10 types of anion-exchange resin were investigated, and the results showed that the 717 anion-exchange resin exhibited the highest capacity of adsorption and desorption to dihydroartemisinic acid. Adsorption isotherms were established for the 717 anion-exchange resin and they fitted well with both Langmuir and Freundlich model. Dynamic adsorption and desorption properties of 717 anion-exchange resin were characterized to optimize the chromatographic conditions. Subsequently, the silica-gel column chromatography was performed and dihydroartemisinic acid with a purity of up to 98% (w/w) was obtained. Finally, the scale-up experiments validated the preparative separation of high-purity dihydroartemisinic acid from industrial waste developed in the present work. This work presented for the first time an isolation of dihydroartemisinic acid with a purity of 98% from Artemisia annua (A. annua) by-product, which adds more value to this crop and has the potential to lower the prices of anti-malarial drugs.
为了充分利用青蒿素生产废料从而降低青蒿素的生产成本，我们开发了一种高效且可扩展的方法，通过将阴离子交换树脂与硅胶柱色谱相结合，从青蒿素生产废料中分离出高纯度的二氢青蒿酸。研究了二氢青蒿酸在10种阴离子交换树脂上的吸附和解吸特性，结果表明717阴离子交换树脂对二氢青蒿酸表现出最高的吸附和解吸能力。建立了717阴离子交换树脂的吸附等温线，其与Langmuir模型和Freundlich模型均拟合良好。对717阴离子交换树脂的动态吸附和解吸性能进行了表征，以优化色谱条件。随后，进行了硅胶柱色谱分离，得到了纯度高达98%（w/w）的二氢青蒿酸。最后，放大实验验证了本工作中从工业废料中制备分离高纯度二氢青蒿酸的方法。这项工作首次从青蒿副产物中分离出纯度为98%的二氢青蒿酸，这为该作物增加了更多价值，并有可能降低抗疟药物的价格。
