Leong Ivone Un San, Lai Daniel, Lan Chuan-Ching, Johnson Ross, Love Donald R, Johnson Ross, Love Donald R
Molecular, Cellular and Developmental Biology Research Section, School of Biological Sciences, University of Auckland, Private Bag, Auckland, New Zealand.
Birth Defects Res C Embryo Today. 2011 Sep;93(3):249-55. doi: 10.1002/bdrc.20213.
The modeling of human disease in the zebrafish (Danio rerio) is moving away from chemical mutagensis and transient downregulation using morpholino oligomers to more targeted and stable transgenic methods. In this respect, zinc finger nucleases offer a means of introducing mutations at targeted sites at high efficiency. We describe here the development of zinc finger nucleases and their general use in model systems with a focus on the zebrafish.
斑马鱼（Danio rerio）中人类疾病的建模正从化学诱变和使用吗啉代寡聚物的瞬时下调转向更具靶向性和稳定性的转基因方法。在这方面，锌指核酸酶提供了一种在靶位点高效引入突变的手段。我们在此描述锌指核酸酶的开发及其在模型系统中的一般应用，重点是斑马鱼。
