Kok Fatma O, Gupta Ankit, Lawson Nathan D, Wolfe Scot A
Program in Gene Function & Expression, Department of Biochemistry & Molecular Pharmacology, UMass Medical School, Worcester, MA, USA.
Methods Mol Biol. 2014;1101:267-303. doi: 10.1007/978-1-62703-721-1_13.
Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.
人工核酸酶已发展成为在多种物种中引入精确基因组修饰的强大工具。在本章中，作者提供了详细的方案，用于快速构建锌指核酸酶（ZFNs）和转录激活样效应因子核酸酶（TALENs），并评估它们在斑马鱼基因组中靶向产生插入缺失的活性。
