Canada Research Chair on Electroactive and Photoactive Polymers, Département de Chimie, Université Laval, Québec City, Québec, Canada.
Anal Chem. 2011 Nov 1;83(21):8086-92. doi: 10.1021/ac200713f. Epub 2011 Oct 3.
We report a new electrochemical amplification strategy for an ultrasensitive electrochemical detection of DNA sequences using aggregates composed of a water-soluble, ferrocene-functionalized polythiophene. A two-step hybridization is performed at one addressing surface with PNA capture probes whereas the electrochemical detection is done on an electrode nearby. Specific and quantitative detection of DNA targets with a detection limit of 4 × 10(-16) M (about 4 zeptomoles or about 2500 copies of oligonucleotides) was achieved.
我们报告了一种新的电化学放大策略，用于使用由水溶性、二茂铁功能化聚噻吩组成的聚集体进行 DNA 序列的超灵敏电化学检测。在一个寻址表面上进行两步杂交，使用 PNA 捕获探针，而电化学检测则在附近的电极上进行。实现了对 DNA 靶标的特异性和定量检测，检测限为 4×10(-16) M（约 4 飞摩尔或约 2500 个寡核苷酸拷贝）。
