利用含二茂铁阳离子聚噻吩的聚集体进行扩增策略，用于 DNA 的灵敏和特异电化学检测。

Amplification strategy using aggregates of ferrocene-containing cationic polythiophene for sensitive and specific electrochemical detection of DNA.

机构信息

Canada Research Chair on Electroactive and Photoactive Polymers, Département de Chimie, Université Laval, Québec City, Québec, Canada.

出版信息

Anal Chem. 2011 Nov 1;83(21):8086-92. doi: 10.1021/ac200713f. Epub 2011 Oct 3.

DOI:10.1021/ac200713f

PMID:21932839

Abstract

We report a new electrochemical amplification strategy for an ultrasensitive electrochemical detection of DNA sequences using aggregates composed of a water-soluble, ferrocene-functionalized polythiophene. A two-step hybridization is performed at one addressing surface with PNA capture probes whereas the electrochemical detection is done on an electrode nearby. Specific and quantitative detection of DNA targets with a detection limit of 4 × 10(-16) M (about 4 zeptomoles or about 2500 copies of oligonucleotides) was achieved.

摘要

我们报告了一种新的电化学放大策略，用于使用由水溶性、二茂铁功能化聚噻吩组成的聚集体进行 DNA 序列的超灵敏电化学检测。在一个寻址表面上进行两步杂交，使用 PNA 捕获探针，而电化学检测则在附近的电极上进行。实现了对 DNA 靶标的特异性和定量检测，检测限为 4×10(-16) M（约 4 飞摩尔或约 2500 个寡核苷酸拷贝）。