Department of Chemistry and Material Science, Hengyang Normal University, Hengyang, Hunan 421008, China.
Talanta. 2012 Jan 15;88:216-21. doi: 10.1016/j.talanta.2011.10.035. Epub 2011 Oct 31.
A polythiophene functionalized with methylene blue (PMT-MB) was synthesized and used as an indicator for electrochemical oligonucleotides (ODNs) hybridization detection. After hybridization with complementary ODNs, the current signal of PMT-MB increased, which illustrated that PMT-MB can effectively recognize complementary ODN targets as an indicator. Compared to MB, PMT-MB showed much better resistance to the concentration change of buffer solution. In all buffer solutions tested, the hybridization always resulted in the increased current signal of PMT-MB due to the electrostatic interaction. While, when MB was used as an indicator, the inconsistent current response was obtained after the hybridization. When high concentration of buffer solution was used for accumulation, the hybridization resulted in the decreased current signal, while at the low concentrations, the current signal increased. The interaction between PMT-MB and dsODNs was also studied by UV-vis spectroscopy.
合成了一种亚甲基蓝功能化聚噻吩（PMT-MB），并将其用作电化学寡核苷酸（ODNs）杂交检测的指示剂。与互补 ODN 杂交后，PMT-MB 的电流信号增加，表明 PMT-MB 可以作为指示剂有效地识别互补的 ODN 靶标。与 MB 相比，PMT-MB 对缓冲溶液浓度变化的抵抗力要强得多。在所有测试的缓冲溶液中，由于静电相互作用，杂交总是导致 PMT-MB 的电流信号增加。然而，当 MB 用作指示剂时，杂交后得到的电流响应不一致。当使用高浓度的缓冲溶液进行积累时，杂交导致电流信号减小，而在低浓度时，电流信号增加。还通过紫外可见光谱研究了 PMT-MB 和 dsODNs 之间的相互作用。
