Department of Chemistry, Syracuse University, Syracuse, NY 13244, USA.
Anal Biochem. 2011 Dec 15;419(2):145-52. doi: 10.1016/j.ab.2011.08.042. Epub 2011 Aug 28.
The use of coenzyme Q10 (CoQ10) has been increasing rapidly during recent years due to its postulated beneficial properties in human health, providing energy and antioxidant protection. There are no known negative side effects of CoQ10 even at very high levels. Recently, native saposin B (sapB) has been shown to bind CoQ10 and subsequently be excreted. It is thought that this interaction between sapB and CoQ10 could be a mechanism to avoid any possible CoQ10 toxicity. The interaction between sapB and CoQ10 is poorly understood. Here we present an increased fermentative yield of recombinant sapB and demonstrate that recombinant sapB will bind CoQ10 in a pH-dependent manner similar to sapB binding with other lipids. SapB was coated onto an IMAC (immobilized metal affinity chromatography) resin and successfully bound CoQ10 at pH 5.0 with release of the CoQ10 at pH 9.0.
近年来，由于辅酶 Q10（CoQ10）在人类健康方面的假定有益特性，提供能量和抗氧化保护，其使用量迅速增加。即使在非常高的水平下，CoQ10 也没有已知的负面副作用。最近，天然脑苷脂 B（sapB）已被证明能结合 CoQ10 并随后被排出。人们认为，sapB 和 CoQ10 之间的这种相互作用可能是一种避免任何可能的 CoQ10 毒性的机制。sapB 和 CoQ10 之间的相互作用还知之甚少。在这里，我们展示了重组 sapB 的发酵产量增加，并证明重组 sapB 将以类似于 sapB 与其他脂质结合的 pH 依赖性方式结合 CoQ10。将 sapB 涂覆到 IMAC（固定金属亲和层析）树脂上，并在 pH 5.0 下成功结合 CoQ10，在 pH 9.0 下释放 CoQ10。
