Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
Poult Sci. 2011 Oct;90(10):2332-41. doi: 10.3382/ps.2011-01596.
The goal of this study was to examine whether lipopolysaccharide (LPS) induces the expression of proinflammatory cytokines and chemokines and recruits T cells in the lower part of the oviduct, and whether that response to LPS is different between the laying and molting phase. White Leghorn laying and molting hens were intravenously injected with saline (control) or LPS. The uterus and vagina of oviducts were collected 3 or 6 h after injection, and used for reverse transcription PCR analysis of IL-1β, IL-6, IL-8 (CXCLi2), and lymphotactin (Lptn), and for immunohistochemical analysis for the frequency of CD4+ and CD8+ T cells. The expressions of IL-1β, IL-6, and CXCLi2 in the uterus and that of IL-1β in the vagina were upregulated in response to LPS 3 or 6 h after injection in both laying and molting hens. The CXCLi2 expression in the vagina was upregulated by LPS in laying hens, whereas those effects of LPS were not significant in molting hens. Expression of Lptn showed a tendency to be downregulated after 3 h, with recovery by 6 h after LPS injection. The frequency of CD4+ T cells tended to increase in response to LPS after 6 h in the lamina propria of the uterus and vagina in both laying and molting hens. The CD8+ T cell frequencies in the lamina propria of the uterus and vagina of laying hens increased in response to LPS after 6 h. However, in the molting hens, LPS stimulation resulted in CD8+ T cell increase in the vagina only and not in the uterus. These results suggest that expressions of proinflammatory cytokines and CXCLi2 chemokine are upregulated in association with T cell recruitment in response to LPS in the lower part of the oviduct, although CD8+ T cells in the uterus may be depressed during the molting phase. These immunoresponses may play roles in the defense against infection of the oviduct.
本研究旨在探讨脂多糖（LPS）是否会在下生殖道诱导促炎细胞因子和趋化因子的表达，并招募 T 细胞，以及 LPS 对这些反应是否在产蛋期和换羽期有所不同。给白来航产蛋和换羽母鸡静脉注射生理盐水（对照）或 LPS。注射后 3 或 6 小时收集输卵管的子宫和阴道，用于 IL-1β、IL-6、IL-8（CXCLi2）和淋球菌趋化因子（Lptn）的逆转录 PCR 分析，以及 CD4+和 CD8+T 细胞的免疫组织化学分析。注射 LPS 后 3 或 6 小时，子宫中 IL-1β、IL-6 和 CXCLi2 的表达以及阴道中 IL-1β的表达在产蛋和换羽母鸡中均上调。LPS 在下生殖道中上调了 CXCLi2 的表达，但在换羽母鸡中这种作用不显著。LPS 注射后 3 小时 Lptn 的表达呈下调趋势，6 小时后恢复。产蛋和换羽母鸡的子宫和阴道固有层中，CD4+T 细胞在 LPS 作用后 6 小时呈增加趋势。然而，在换羽母鸡中，LPS 刺激仅导致阴道中的 CD8+T 细胞增加，而子宫中则没有。这些结果表明，在下生殖道中，LPS 诱导促炎细胞因子和趋化因子表达与 T 细胞募集相关，尽管在换羽期，子宫中的 CD8+T 细胞可能会减少。这些免疫反应可能在输卵管抗感染中发挥作用。
