Sodeoka Live Cell Chemistry Project, ERATO, Japan Science and Technology, Wako-shi, Saitama 351-0198, Japan.
Org Biomol Chem. 2011 Oct 26;9(22):7667-70. doi: 10.1039/c1ob06123b. Epub 2011 Sep 20.
A cobalt-phosphine complex supported on PS-PEG beads was found to react with a propargyl carbamate tag, and the tagged molecules immobilized on the beads could be released by acidic treatment through the Nicholas reaction pathway. These reactions work in aqueous media at 4 °C, so that this catch and release procedure is compatible with conditions generally used in biochemical experiments.
一种负载在 PS-PEG 珠上的钴-膦配合物被发现能与炔丙基碳酸酯标签反应，而固定在珠上的标记分子可以通过 Nicholas 反应途径在酸性条件下释放。这些反应在 4°C 的水相介质中进行，因此这种捕获和释放过程与生化实验中通常使用的条件兼容。
