Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, Portugal.
Mol Nutr Food Res. 2011 Nov;55(11):1735-9. doi: 10.1002/mnfr.201100287. Epub 2011 Sep 21.
We assessed the impact of oligofructose (OFS) and dextrin (DEX) as diet supplements on hepatic redox state. Rats were fed either a 10% OFS or a 10% DEX supplemented diet for 9 wk. In the DEX diet group, the levels of hepatic protein carbonylation were decreased by 63%. Total glutathione and reduced glutathione (GSH) contents were reduced in the OFS and DEX diet groups by around 20%. DEX supplementation significantly reduced oxidized glutathione (GSSG) levels resulting in a 33% increase in the GSH/GSSG ratio. The activity of the hepatic antioxidant enzymes was not changed by either OFS or DEX supplementation. OFS supplementation caused a decrease in serum levels of triglycerides (36%), cholesterol (24%), HDL (16%) and LDL (17%). DEX supplementation only reduced triglycerides (32%) and urea (22%). Both diets increased serum levels of acetate by fivefold and propionate by twofold, but DEX diet decreased butyrate levels by 75%. Due to their different composition/structure these two dietary fibers affected metabolism in different ways. Diet supplementation with 10% DEX can potentially improve host health, by protecting the liver from protein carbonylation and by improving GSH/GSSG ratio and diet supplementation with 10% OFS can improve the lipid profile.
我们评估了低聚果糖（OFS）和糊精（DEX）作为饮食补充剂对肝脏氧化还原状态的影响。大鼠分别喂食 10%的 OFS 或 DEX 补充饮食 9 周。在 DEX 饮食组中，肝蛋白羰基化水平降低了 63%。OFS 和 DEX 饮食组的总谷胱甘肽和还原型谷胱甘肽（GSH）含量降低了约 20%。DEX 补充显著降低了氧化型谷胱甘肽（GSSG）水平，导致 GSH/GSSG 比值增加 33%。OFS 或 DEX 补充均未改变肝抗氧化酶的活性。OFS 补充导致血清甘油三酯（36%）、胆固醇（24%）、HDL（16%）和 LDL（17%）水平降低。DEX 补充仅降低了甘油三酯（32%）和尿素（22%）。两种饮食均使血清乙酸盐水平增加了五倍，丙酸盐增加了两倍，但 DEX 饮食使丁酸盐水平降低了 75%。由于它们的组成/结构不同，这两种膳食纤维以不同的方式影响代谢。饮食补充 10%的 DEX 可以通过保护肝脏免受蛋白羰基化，改善 GSH/GSSG 比值来改善宿主健康，而饮食补充 10%的 OFS 可以改善血脂谱。
