Department of Mechanical Engineering, National Yunlin University of Science and Technology, Taiwan.
J Biomech. 2011 Nov 10;44(16):2790-4. doi: 10.1016/j.jbiomech.2011.08.021. Epub 2011 Sep 19.
The morphological change of cellular apoptosis initiates from the change of membrane roughness. In order to identify cellular apoptosis in its early stage, atomic force microscope was adapted to reveal the change of membrane roughness in unprecedented details, providing an image in nanometer-scaled resolution. The mouse monocyte/macrophage cell line RAW 264.7 was the subject studied and subjected to apoptotic induction by hydrogen peroxide. A finding of the qualitative correlation between cell membrane roughness and oxidative stress level is disclosed stating that roughness is increasing with the increasing level of oxidative stress.
细胞凋亡的形态变化始于膜粗糙度的变化。为了在早期识别细胞凋亡，原子力显微镜被用来以前所未有的细节揭示膜粗糙度的变化，提供纳米级分辨率的图像。以小鼠单核细胞/巨噬细胞系 RAW 264.7 为研究对象，用双氧水诱导其凋亡。结果揭示了细胞膜粗糙度与氧化应激水平之间存在定性相关性，即随着氧化应激水平的增加，粗糙度也随之增加。
