Institute of Hematology, Medical College, Jinan University, No. 601, West Huangpu Road, Guangzhou 510632, China.
Med Oncol. 2013 Mar;30(1):430. doi: 10.1007/s12032-012-0430-6. Epub 2013 Jan 10.
Bcl-2 is a prominent member of the Bcl-2 family of proteins that regulate the induction of apoptosis. Here, we investigated the effect of Bcl-2 small interfering RNA (siRNA) combined with miR-15a oligonucleotides (ODN) on the growth of Raji cells. The expression levels of Bcl-2 mRNA and protein were detected. The inhibition of cell growth and apoptosis was determined. Atomic force microscopy was utilized to observe the cell-surface ultrastructural changes. After transfection of combination, protein and mRNA levels of Bcl-2 obviously were decreased. The growth of cells was significantly inhibited compared with those cells transfected with Bcl-2 siRNA or miR-15a alone. Apoptotic rate significantly increased. The cells of apoptosis showed a roughness variation on cell membranes. These results suggest that the combination of Bcl-2 siRNA and miR-15a ODN increases apoptosis in Raji cells. Our study implies that the combination of Bcl-2 siRNA and miR-15a may be a useful approach in treatment for lymphoma.
Bcl-2 是 Bcl-2 蛋白家族的重要成员，可调节细胞凋亡的诱导。在这里，我们研究了 Bcl-2 小干扰 RNA（siRNA）与 miR-15a 寡核苷酸（ODN）联合对 Raji 细胞生长的影响。检测了 Bcl-2 mRNA 和蛋白的表达水平，确定了细胞生长抑制和凋亡的情况。原子力显微镜用于观察细胞表面超微结构的变化。转染后，Bcl-2 的蛋白和 mRNA 水平明显降低。与单独转染 Bcl-2 siRNA 或 miR-15a 的细胞相比，细胞生长明显受到抑制，凋亡率显著增加。凋亡细胞的细胞膜表现出粗糙度变化。这些结果表明，Bcl-2 siRNA 和 miR-15a ODN 的联合可增加 Raji 细胞的凋亡。我们的研究表明，Bcl-2 siRNA 和 miR-15a 的联合可能是淋巴瘤治疗的一种有效方法。
