Deichman G, Kashkina L, Rapp F
Neoplasma. 1979;26(1):49-56.
Newborn and adult Syrian hamsters were injected with wild-type SV40 and its temperature-sensitive (ts) mutants A30, A209, A239, B201 and BC210. In contrast to wild-type SV40, ts A30, ts A 239 and ts BC210 were oncogenic in adult hamsters inducing tumors after almost the same latent period as wild type SV40 in newborns but in lower number of animals. Study of TSTA in some tumors (1 generation) induced in newborn and adult hamsters by wild type SV40 and its ts mutants (with the use of immunogenicity and immunosensitivity tests) revealed no significant difference among compared tumors: most of them were immunogenic and immunosensitive. In contrast hamster embryo cells in vitro transformed by SV40 ts A30, ts A239 and ts A209 mutants, studied at different passage levels were all immunoresistant during about 30 in vitro passages and in most cases 10--100 times less immunogenic than hamster embryo cells in vitro transformed by wild type SV40. At higher passage level in some of these lines expression of TSTA improved. The data obtained are discussed in connection with the recently demonstrated [5] significant quantitative difference in tumor specific transplantation antigen activity in hamster cells in vivo and vitro infected, or transformed by wild type SV40 and its ts A mutants.
将野生型SV40及其温度敏感（ts）突变体A30、A209、A239、B201和BC210注射到新生和成年叙利亚仓鼠体内。与野生型SV40不同，ts A30、ts A 239和ts BC210在成年仓鼠中具有致癌性，诱导肿瘤形成的潜伏期与野生型SV40在新生仓鼠中的潜伏期几乎相同，但诱发肿瘤的动物数量较少。通过野生型SV40及其ts突变体在新生和成年仓鼠中诱导产生的一些肿瘤（第1代）进行肿瘤特异性移植抗原（TSTA）研究（使用免疫原性和免疫敏感性试验），结果显示，所比较的肿瘤之间没有显著差异：大多数肿瘤具有免疫原性且对免疫敏感。相比之下，用SV40 ts A30、ts A239和ts A209突变体体外转化的仓鼠胚胎细胞，在不同传代水平进行研究时，在大约30次体外传代过程中均具有免疫抗性，并且在大多数情况下，其免疫原性比用野生型SV40体外转化的仓鼠胚胎细胞低10 - 100倍。在这些细胞系中的一些细胞系传代水平较高时，TSTA的表达有所改善。结合最近证明的[5]野生型SV40及其ts A突变体感染或转化的仓鼠体内和体外细胞中肿瘤特异性移植抗原活性存在显著定量差异，对所获得的数据进行了讨论。
