Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias and BIFI, Universidad de Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain.
J Plant Physiol. 2011 Dec 15;168(18):2244-50. doi: 10.1016/j.jplph.2011.08.006. Epub 2011 Sep 21.
Ferric uptake regulation (Fur) proteins are prokaryotic transcriptional regulators that integrate signaling of iron metabolism and oxidative stress responses with several environmental stresses. In photosynthetic organisms, Fur proteins regulate many genes involved in photosynthesis, nitrogen metabolism and other key processes. Also, Fur triggers the expression of virulence factors in many bacterial pathogens, and Fur from Microcystis aeruginosa has been shown to bind promoter regions of the microcystin synthesis gene cluster. In this work, we studied transcriptional responses of fur genes under different light intensities and oxidative stress. An antisense of fur, the α-fur RNA, plays an important role in regulating fur expression under oxidative stress, affecting levels of Fur protein in cells. Importantly, an active photosynthetic electron chain is required for the expression of the fur gene.
铁摄取调节（Fur）蛋白是原核转录调节因子，可将铁代谢信号与氧化应激反应以及几种环境应激整合在一起。在光合生物中，Fur 蛋白调节许多与光合作用、氮代谢和其他关键过程相关的基因。此外，Fur 还触发许多细菌病原体中毒力因子的表达，并且已表明铜绿微囊藻中的 Fur 蛋白结合微囊藻毒素合成基因簇的启动子区域。在这项工作中，我们研究了在不同光强和氧化应激下 fur 基因的转录反应。fur 的反义 RNA（α-fur RNA）在氧化应激下调节 fur 表达中起重要作用，影响细胞中 Fur 蛋白的水平。重要的是，活性光合作用电子链是 fur 基因表达所必需的。
