整合素连接激酶在胃癌细胞黏附、侵袭和肿瘤生长中的作用。
Integrin-linked kinase in gastric cancer cell attachment, invasion and tumor growth.
机构信息
Department of General Surgery, Beijing Hospital, Peking University, 100730 Beijing, China.
出版信息
World J Gastroenterol. 2011 Aug 14;17(30):3487-96. doi: 10.3748/wjg.v17.i30.3487.
AIM
To investigate the effects of integrin-linked kinase (ILK) on gastric cancer cells both in vitro and in vivo.
METHODS
ILK small interfering RNA (siRNA) was transfected into human gastric cancer BGC-823 cells and ILK expression was monitored by real-time quantitative polymerase chain reaction, Western blotting analysis and immunocytochemistry. Cell attachment, proliferation, invasion, microfilament dynamics and the secretion of vascular endothelial growth factor (VEGF) were also measured. Gastric cancer cells treated with ILK siRNA were subcutaneously transplanted into nude mice and tumor growth was assessed.
RESULTS
Both ILK mRNA and protein levels were significantly down-regulated by ILK siRNA in human gastric cancer cells. This significantly inhibited cell attachment, proliferation and invasion. The knockdown of ILK also disturbed F-actin assembly and reduced VEGF secretion in conditioned medium by 40% (P < 0.05). Four weeks after injection of ILK siRNA-transfected gastric cancer cells into nude mice, tumor volume and weight were significantly reduced compared with that of tumors induced by cells treated with non-silencing siRNA or by untreated cells (P < 0.05).
CONCLUSION
Targeting ILK with siRNA suppresses the growth of gastric cancer cells both in vitro and in vivo. ILK plays an important role in gastric cancer progression.
目的
研究整合素连接激酶（ILK）在体外和体内对胃癌细胞的影响。
方法
将 ILK 小干扰 RNA（siRNA）转染入人胃癌 BGC-823 细胞，通过实时定量聚合酶链反应、Western 印迹分析和免疫细胞化学监测 ILK 表达。还测量了细胞黏附、增殖、侵袭、微丝动力学和血管内皮生长因子（VEGF）的分泌。用 ILK siRNA 处理的胃癌细胞被皮下移植到裸鼠中，并评估肿瘤生长。
结果
ILK siRNA 可显著下调人胃癌细胞中的 ILK mRNA 和蛋白水平。这显著抑制了细胞黏附、增殖和侵袭。ILK 的敲低还扰乱了 F-肌动蛋白组装，并使条件培养基中的 VEGF 分泌减少了 40%（P < 0.05）。将 ILK siRNA 转染的胃癌细胞注射入裸鼠 4 周后，与用非沉默 siRNA 处理的细胞或未经处理的细胞诱导的肿瘤相比，肿瘤体积和重量显著减小（P < 0.05）。
结论
用 siRNA 靶向 ILK 可抑制胃癌细胞在体外和体内的生长。ILK 在胃癌进展中起重要作用。
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