Department of General Surgery, Shengjing Hospital, China Medical University, 36 Sanhao Street, Shenyang 110004, People's Republic of China.
J Mol Histol. 2013 Apr;44(2):183-9. doi: 10.1007/s10735-012-9463-6. Epub 2012 Oct 31.
Integrin-linked kinase (ILK), an intracellular serine-threonine kinase, has been reported to be overexpressed in multiple types of human malignancies, including colorectal cancer (CRC). The prognostic value of ILK in CRC, however, remains unknown. In the present study, expression of ILK in 25 paired primary CRC samples and adjacent noncancerous tissues were quantified using real-time PCR and Western blotting. ILK protein expression was analyzed in 102 archived, paraffin-embedded CRC samples using immunohistochemistry. The correlation between ILK expression and clinicopathological factors was evaluated by the χ(2) test. Patients' overall survival was analyzed by Kaplan-Meier method. We found that both ILK mRNA and protein expression levels were significantly up-regulated in primary CRC samples compared with their corresponding normal tissues. Immunohistochemical analysis revealed relative high expression of ILK in 43 of 102 (42.2 %) primary CRC samples. Statistical analysis showed a significant correlation of ILK expression with tumor differentiation, lymph node metastasis, tumor invasion, and tumor-node-metastasis stage. Patients with tumors displaying high-level ILK expression showed significantly shorter overall survival (P = 0.028, log-rank test). More importantly, multivariate analysis indicated that high ILK protein expression was an independent prognostic factor for CRC patients (P = 0.026). Taken together, our data suggest that ILK overexpression is associated with tumor progression and a poor prognosis in CRC patients and may represent a novel potential prognostic marker for patients with CRC.
整合素连接激酶（ILK）是一种细胞内丝氨酸-苏氨酸激酶，已被报道在多种人类恶性肿瘤中过表达，包括结直肠癌（CRC）。然而，ILK 在 CRC 中的预后价值尚不清楚。在本研究中，使用实时 PCR 和 Western blot 定量检测了 25 对原发性 CRC 样本及其相邻非癌组织中 ILK 的表达。使用免疫组织化学分析了 102 个存档的石蜡包埋 CRC 样本中的 ILK 蛋白表达。通过 χ(2)检验评估 ILK 表达与临床病理因素之间的相关性。通过 Kaplan-Meier 方法分析患者的总生存情况。我们发现，与相应的正常组织相比，原发性 CRC 样本中的 ILK mRNA 和蛋白表达水平均显著上调。免疫组织化学分析显示，在 102 个原发性 CRC 样本中，有 43 个（42.2%）显示相对高表达的 ILK。统计分析显示，ILK 表达与肿瘤分化、淋巴结转移、肿瘤侵袭和肿瘤-淋巴结-转移分期显著相关。显示高水平 ILK 表达的患者总生存时间明显缩短（P=0.028，log-rank 检验）。更重要的是，多变量分析表明，高 ILK 蛋白表达是 CRC 患者的独立预后因素（P=0.026）。综上所述，我们的数据表明，ILK 过表达与 CRC 患者的肿瘤进展和不良预后相关，可能代表 CRC 患者的一个新的潜在预后标志物。
