Department of Neurosurgery, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Shenyang, 110001, Liaoning, People's Republic of China.
J Mol Histol. 2014 Apr;45(2):141-51. doi: 10.1007/s10735-013-9540-5. Epub 2013 Sep 13.
Integrin-linked kinase (ILK) is a ubiquitously expressed serine/threonine protein kinase that has been implicated in cancer development, progression and metastasis. The aim of the present study was to characterize the role of ILK in glioma cell invasion and migration. We generated a recombinant eukaryotic expression vector containing the human ILK gene and transfected it into human glioma SHG-44 cells. Real-time PCR and western blot analysis were used to identify the stable transformants. The wound healing and Transwell invasion assays showed that ectopic overexpression of ILK in SHG-44 cells significantly promoted their migration and invasion capabilities in culture. This was accompanied by a decrease in expression of E-cadherin and an increase in expression of Snail and Slug. Moreover, the decrease in E-cadherin expression induced by ILK overexpression was greatly restored by the nuclear factor-κB (NF-κB) inhibitor BAY 11-7028 or small interfering RNA targeting NF-κB p65, indicating an involvement of NF-κB in ILK-induced down-regulation of E-cadherin. In conclusion, our data underscore a novel role for ILK in glioma invasion and metastasis processes, implicating potential for therapeutic interference.
整合素连接激酶（ILK）是一种普遍表达的丝氨酸/苏氨酸蛋白激酶，已被牵涉到癌症的发生、发展和转移中。本研究旨在探讨 ILK 在神经胶质瘤细胞侵袭和迁移中的作用。我们构建了一个包含人 ILK 基因的重组真核表达载体，并将其转染入人神经胶质瘤 SHG-44 细胞中。实时 PCR 和 Western blot 分析用于鉴定稳定转染的细胞。划痕愈合和 Transwell 侵袭实验表明，ILK 在 SHG-44 细胞中的异位过表达显著促进了它们在培养中的迁移和侵袭能力。这伴随着 E-钙黏蛋白表达的降低和 Snail 和 Slug 表达的增加。此外，ILK 过表达诱导的 E-钙黏蛋白表达降低，通过核因子-κB（NF-κB）抑制剂 BAY 11-7028 或靶向 NF-κB p65 的小干扰 RNA 得到了极大的恢复，表明 NF-κB 参与了 ILK 诱导的 E-钙黏蛋白下调。总之，我们的数据强调了 ILK 在神经胶质瘤侵袭和转移过程中的新作用，暗示了治疗干预的潜力。
