Shishatskaya Ekaterina I, Nikolaeva Elena D, Vinogradova Olga N, Volova Tatiana G
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, 50-50 Akademgorodok, Krasnoyarsk, 660036, Russia.
Siberian Federal University, 79 Svobodniy Ave., Krasnoyarsk, 660041, Russia.
J Mater Sci Mater Med. 2016 Nov;27(11):165. doi: 10.1007/s10856-016-5776-4. Epub 2016 Sep 21.
The present study reports construction of wound dressing materials from degradable natural polymers such as hydroxy derivatives of carboxylic acids (PHAs) and 3-hydroxybutyrate/4-hydroxybutyrate [P(3HB/4HB)] as copolymer. The developed polymer films and electrospun membranes were evaluated for its wound healing properties with Grafts-elastic nonwoven membranes carrying fibroblast cells derived from adipose tissue multipotent mesenchymal stem cells. The efficacy of nonwoven membranes of P(3HB/4HB) carrying the culture of allogenic fibroblasts was assessed against model skin defects in Wistar rats. The morphological, histological and molecular studies revealed the presence of fibroblasts on dressing materials which facilitated wound healing, vascularization and regeneration. Further it was also observed that cells secreted extracellular matrix proteins which formed a layer on the surface of membranes and promoted the migration of epidermal cells from the neighboring tissues surrounding the wound. The wounds under the P(3HB/4HB) membrane carrying cells healed 1.4 times faster than the wounds under the cell-free membrane and 3.5 times faster than the wounds healing under the eschar (control).The complete wound healing process was achieved at Day 14. Thus the study highlights the importance of nonwoven membranes developed from degradable P(3HB/4HB) polymers in reducing inflammation, enhancing angiogenic properties of skin and facilitating better wound healing process.
本研究报告了由可降解天然聚合物构建伤口敷料材料的情况，这些聚合物如羧酸的羟基衍生物（PHA）和作为共聚物的3-羟基丁酸酯/4-羟基丁酸酯[P(3HB/4HB)]。用携带源自脂肪组织多能间充质干细胞的成纤维细胞的移植弹性非织造膜对所开发的聚合物薄膜和电纺膜的伤口愈合特性进行了评估。评估了携带同种异体成纤维细胞培养物的P(3HB/4HB)非织造膜对Wistar大鼠模型皮肤缺损的疗效。形态学、组织学和分子研究表明，敷料材料上存在成纤维细胞，这促进了伤口愈合、血管形成和再生。此外，还观察到细胞分泌细胞外基质蛋白，这些蛋白在膜表面形成一层，并促进表皮细胞从伤口周围的邻近组织迁移。携带细胞的P(3HB/4HB)膜下的伤口愈合速度比无细胞膜下的伤口快1.4倍，比焦痂（对照）下的伤口愈合速度快3.5倍。在第14天实现了完全的伤口愈合过程。因此，该研究突出了由可降解的P(3HB/4HB)聚合物开发的非织造膜在减轻炎症、增强皮肤血管生成特性和促进更好的伤口愈合过程中的重要性。
