Department of Otolaryngology-Head and Neck Surgery, University of Yamanashi, Chuo, Yamanashi, Japan.
Cell Immunol. 2011;271(2):488-95. doi: 10.1016/j.cellimm.2011.08.019. Epub 2011 Aug 31.
Cedar pollens cause severe allergic disease throughout the world. We have previously characterized allergenic pollen glycoproteins from mountain cedar (Juniperus ashei) that bind to allergen-specific immunoglobulin E (IgE). In the present report, we investigated an alternative pathway of mast cell activation by mountain cedar pollen extract through IgE-independent mechanisms. We show that mountain cedar pollen directly induces mast cell serotonin and IL-4 release and enhances release induced by IgE cross-linking. Concomitant with mediator release, high levels of intracellular reactive oxygen species (ROS) were generated, and both ROS and serotonin release were inhibited by anti-oxidants. These findings suggest that alternative mechanisms exist whereby pollen exposure enhances allergic inflammatory mediator release through mechanisms that involve ROS. These mechanisms have the potential for enhancing the allergenic potency of pollens.
雪松花粉在全世界范围内引起严重的过敏疾病。我们之前已经从西部红柏（Juniperus ashei）中鉴定出了与过敏原特异性免疫球蛋白 E（IgE）结合的过敏花粉糖蛋白。在本报告中，我们通过 IgE 非依赖性机制研究了西部红柏花粉提取物激活肥大细胞的另一种途径。我们表明，西部红柏花粉可直接诱导肥大细胞释放血清素和白细胞介素-4，并增强 IgE 交联诱导的释放。伴随介质释放，会产生高水平的细胞内活性氧物质（ROS），并且抗氧化剂可以抑制 ROS 和血清素的释放。这些发现表明，花粉暴露通过涉及 ROS 的机制增强过敏炎症介质释放存在替代机制。这些机制有可能增强花粉的变应原效力。
