Rakugi H, Tabuchi Y, Nakamaru M, Nagano M, Higashimori K, Mikami H, Ogihara T, Suzuki N
Department of Geriatric Medicine, Osaka University Medical School, Japan.
Biochem Biophys Res Commun. 1990 Jun 29;169(3):973-7. doi: 10.1016/0006-291x(90)91989-6.
To elucidate further the contribution of endothelin into endothelium-dependent vasoconstriction evoked by hypoxia, we observed endothelin release during hypoxia. Endothelin was detectable in perfusate from mesenteric artery. Immunoreactive endothelin was confirmed as endothelin-1 by a reverse phase-HPLC. Endothelin release increased 4.1 +/- 1.3 to 12.4 +/- 2.0 pg/30 min without changing perfusion pressure. Thirty minutes of hypoxia stimulated endothelin release by 71 +/- 11% (P less than 0.05) and was associated with an elevation of perfusion pressure. These results suggest that endothelin contributes to endothelium-dependent vasoconstriction by hypoxia in mesenteric artery and may play an important role in the local peripheral vascular tone.
为了进一步阐明内皮素在缺氧诱发的内皮依赖性血管收缩中的作用，我们观察了缺氧过程中内皮素的释放情况。在肠系膜动脉灌流液中可检测到内皮素。通过反相高效液相色谱法证实免疫反应性内皮素为内皮素-1。内皮素释放量从4.1±1.3 pg/30分钟增加到12.4±2.0 pg/30分钟，而灌注压力未发生变化。30分钟的缺氧刺激内皮素释放增加了71±11%（P<0.05），并伴有灌注压力升高。这些结果表明，内皮素在肠系膜动脉缺氧诱发的内皮依赖性血管收缩中起作用，可能在局部外周血管张力中发挥重要作用。
